GFP-complementation assay to detect functional CPP and protein delivery into living cells.
AuthorsMilech N, Longville BA, Cunningham PT, Scobie MN, Bogdawa HM, Winslow S, Anastasas M, Connor T, Ong F, Stone SR, Kerfoot M, Heinrich T, Kroeger KM, Tan YF, Hoffmann K, Thomas WR, Watt PM, Hopkins RM,
Journal
PubMed ID26671759
'Efficient cargo uptake is essential for cell-penetrating peptide (CPP) therapeutics, which deliver widely diverse cargoes by exploiting natural cell processes to penetrate the cell''s membranes. Yet most current CPP activity assays are hampered by limitations in assessing uptake, including confounding effects of conjugated fluorophores or ligands, indirect read-outs requiring secondary ... More
BET inhibitor OTX015 targets BRD2 and BRD4 and decreases c-MYC in acute leukemia cells.
AuthorsCoudé MM, Braun T, Berrou J, Dupont M, Bertrand S, Masse A, Raffoux E, Itzykson R, Delord M, Riveiro ME, Herait P, Baruchel A, Dombret H, Gardin C,
Journal
PubMed ID25989842
The bromodomain (BRD) and extraterminal (BET) proteins including BRD2, BRD3 and BRD4 have been identified as key targets for leukemia maintenance. A novel oral inhibitor of BRD2/3/4, the thienotriazolodiazepine compound OTX015, suitable for human use, is available. Here we report its biological effects in AML and ALL cell lines and ... More
Hypomyelinating leukodystrophy-associated missense mutation in HSPD1 blunts mitochondrial dynamics.
AuthorsMiyamoto Y, Eguchi T, Kawahara K, Hasegawa N, Nakamura K, Funakoshi-Tago M, Tanoue A, Tamura H, Yamauchi J,
Journal
PubMed ID25957474
Myelin-forming glial cells undergo dynamic morphological changes in order to produce mature myelin sheaths with multiple layers. In the central nervous system (CNS), oligodendrocytes differentiate to insulate neuronal axons with myelin sheaths. Myelin sheaths play a key role in homeostasis of the nervous system, but their related disorders lead not ... More
Simultaneous Assessment of Clearance, Metabolism, Induction, and Drug-Drug Interaction Potential Using a Long-Term In Vitro Liver Model for a Novel Hepatitis B Virus Inhibitor.
AuthorsKratochwil NA, Triyatni M, Mueller MB, Klammers F, Leonard B, Turley D, Schmaler J, Ekiciler A, Molitor B, Walter I, Gonsard PA, Tournillac CA, Durrwell A, Marschmann M, Jones R, Ullah M, Boess F, Ottaviani G, Jin Y, Parrott NJ, Fowler S
JournalJ Pharmacol Exp Ther
PubMed ID29453199
'Long-term in vitro liver models are now widely explored for human hepatic metabolic clearance prediction, enzyme phenotyping, cross-species metabolism, comparison of low clearance drugs, and induction studies. Here, we present studies using a long-term liver model, which show how metabolism and active transport, drug-drug interactions, and enzyme induction in healthy ... More
Heterogeneity of calcium clock functions in dormant, dysrhythmically and rhythmically firing single pacemaker cells isolated from SA node.
AuthorsKim MS, Maltsev AV, Monfredi O, Maltseva LA, Wirth A, Florio MC, Tsutsui K, Riordon DR, Parsons SP, Tagirova S, Ziman BD, Stern MD, Lakatta EG, Maltsev VA
JournalCell Calcium
PubMed ID30092494
'Current understanding of how cardiac pacemaker cells operate is based mainly on studies in isolated single sinoatrial node cells (SANC), specifically those that rhythmically fire action potentials similar to the in vivo behavior of the intact sinoatrial node. However, only a small fraction of SANC exhibit rhythmic firing after isolation. ... More
Characterization of INCB086550: A Potent and Novel Small-Molecule PD-L1 Inhibitor.
Authors
JournalCancer Discov
PubMed ID35254416
TIRC7 inhibits Th1 cells by upregulating the expression of CTLA-4 and STAT3 in mice with acute graft-versus-host disease.
AuthorsZhu F, Qiu T, Zhu S, Zhao K, Chen C, Qiao J, Pan B, Yan Z, Chen W, Liu Q, Wu Q, Cao J, Sang W, Zeng L, Sun H, Li Z, Xu K
JournalOncol Rep
PubMed ID32319655
'In a previous study, it was demonstrated that T-cell immune response cDNA 7 (TIRC7) levels reflect the efficacy of treatment of patients with acute graft-versus-host disease (GVHD). However, the pathogenesis of TIRC7 in acute GVHD remains poorly understood. Lymphocytes from patients with acute GVHD were selected as targeT cells, and the effects of TIRC7 ... More
Role of Mitochondrial Dynamics in Neuronal Development: Mechanism for Wolfram Syndrome.
AuthorsCagalinec M, Liiv M, Hodurova Z, Hickey MA, Vaarmann A, Mandel M, Zeb A, Choubey V, Kuum M, Safiulina D, Vasar E, Veksler V, Kaasik A,
JournalPLoS Biol
PubMed ID27434582
'Deficiency of the protein Wolfram syndrome 1 (WFS1) is associated with multiple neurological and psychiatric abnormalities similar to those observed in pathologies showing alterations in mitochondrial dynamics. The aim of this study was to examine the hypothesis that WFS1 deficiency affects neuronal function via mitochondrial abnormalities. We show that down-regulation ... More
Wilms Tumor 1b defines a wound-specific sheath cell subpopulation associated with notochord repair.
Authors
JournalElife
PubMed ID29405914
Prophylactic and therapeutic antileukemic effects induced by the AAC-11-derived Peptide RT53.
Authors
JournalOncoimmunology
PubMed ID32158621
Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models.
Authors
JournalNat Methods
PubMed ID35577958
HLA-DR15 Molecules Jointly Shape an Autoreactive T Cell Repertoire in Multiple Sclerosis.
Authors
JournalCell
PubMed ID33091337
Fatty Acid Supplementation Affects Skin Wound Healing in a Rat Model.
Authors
JournalNutrients
PubMed ID35684045
CD8+ T Cell Immunity Is Compromised by Anti-CD20 Treatment and Rescued by Interleukin-17A.
Authors
JournalMBio
PubMed ID32398312
Biphasic responses of human vascular smooth muscle cells to magnesium ion.
AuthorsMa J, Zhao N, Zhu D,
JournalJ Biomed Mater Res A
PubMed ID26402437
Magnesium-based alloys are promising in biodegradable cardiovascular stent applications. The degradation products of magnesium stents may have significant impacts on the surrounding vascular cells. However, knowledge on the interactions between magnesium ion and vascular cells at the molecular and cellular levels is still largely missing. Vascular smooth muscle cell (SMC) ... More
AuthorsWei Z, Yan L, Chen Y, Bao C, Deng J, Deng J,
JournalMol Med Rep
PubMed ID27277156
Mangiferin is a natural polyphenol and the predominant effective component of Mangifera indica Linn. leaves. For hundreds of years, Mangifera indica Linn. leaf has been used as an ingredient in numerous traditional Chinese medicine preparations for the treatment of bronchitis. However, the pharmacological mechanism of mangiferin in the treatment of ... More
Data supporting mitochondrial morphological changes by SPG13-associated HSPD1 mutants.
AuthorsMiyamoto Y, Megumi FT, Hasegawa N, Eguchi T, Tanoue A, Tamura H, Yamauchi J,
JournalData Brief
PubMed ID26900593
The data is related to the research article entitled
Rabex-5 is a lenalidomide target molecule that negatively regulates TLR-induced type 1 IFN production.
AuthorsMillrine D, Tei M, Gemechu Y, Kishimoto T,
JournalProc Natl Acad Sci U S A
PubMed ID27601648
Immunomodulatory drugs (IMiDs) are a family of compounds derived from thalidomide. Binding of the IMiD molecule to the Lon protease Cereblon initiates the degradation of substrates via the ubiquitin proteasome pathway. Here, we show that Cereblon forms a complex with Rabex-5, a regulator of immune homeostasis. Treatment with lenalidomide prevented ... More
Pathogenesis of Anti-PIT-1 Antibody Syndrome: PIT-1 Presentation by HLA Class I on Anterior Pituitary Cells.
AuthorsKanie K, Bando H, Iguchi G, Muguruma K, Matsumoto R, Hidaka-Takeno R, Okimura Y, Yamamoto M, Fujita Y, Fukuoka H, Yoshida K, Suda K, Nishizawa H, Ogawa W, Takahashi Y
JournalJ Endocr Soc
PubMed ID31620667
Anti-pituitary-specific transcriptional factor-1 (anti-PIT-1) antibody syndrome is characterized by acquired and specific deficiencies in growth hormone, prolactin, and thyroid-stimulating hormone. Although PIT-1-reactive cytotoxic T lymphocytes (CTLs) have been speculated to recognize anterior pituitary cells and to cause the injury in the pathogenesis of the syndrome, it remains unclear whether endogenous ... More
Loss of AMPKalpha1 Triggers Centrosome Amplification via PLK4 Upregulation in Mouse Embryonic Fibroblasts.
AuthorsZhao Q, Coughlan KA, Zou MH, Song P
JournalInt J Mol Sci
PubMed ID32316320
Recent evidence indicates that activation of adenosine monophosphate-activated protein kinase (AMPK), a highly conserved sensor and modulator of cellular energy and redox, regulates cell mitosis. However, the underlying molecular mechanisms for AMPKa subunit regulation of chromosome segregation remain poorly understood. This study aimed to ascertain if AMPKa1 deletion contributes to ... More