Cooperativity among Rev-Associated Nuclear Export Signals Regulates HIV-1 Gene Expression and Is a Determinant of Virus Species Tropism.
AuthorsAligeti M, Behrens RT, Pocock GM, Schindelin J, Dietz C, Eliceiri KW, Swanson CM, Malim MH, Ahlquist P, Sherer NM,
Journal
PubMed ID25275125
'Murine cells exhibit a profound block to HIV-1 virion production that was recently mapped to a species-specific structural attribute of the murine version of the chromosomal region maintenance 1 (mCRM1) nuclear export receptor and rescued by the expression of human CRM1 (hCRM1). In human cells, the HIV-1 Rev protein recruits ... More
Discovery of Enzyme Modulators via High-Throughput Time-Resolved FRET in Living Cells.
AuthorsGruber SJ, Cornea RL, Li J, Peterson KC, Schaaf TM, Gillispie GD, Dahl R, Zsebo KM, Robia SL, Thomas DD,
Journal
PubMed ID24436077
'We have used a '
Veterinary services in disasters and emergencies.
AuthorsTennyson AV,
JournalMil Med
PubMed ID2493606
'Potential man-made or natural disasters could overwhelm the residual medical and surgical capacity. Veterinarians have the training and experience to augment physicians in caring for human casualties, and dispersed veterinary hospitals constitute survivable facilities that are equipped to provide medical and surgical care. Veterinarians can also serve public health and ... More
Small-Molecule Probes Targeting the Viral PPxY-Host Nedd4 Interface Block Egress of a Broad Range of RNA Viruses.
AuthorsHan Z, Lu J, Liu Y, Davis B, Lee MS, Olson MA, Ruthel G, Freedman BD, Schnell MJ, Wrobel JE, Reitz AB, Harty RN,
Journal
PubMed ID24741084
'Budding of filoviruses, arenaviruses, and rhabdoviruses is facilitated by subversion of host proteins, such as Nedd4 E3 ubiquitin ligase, by viral PPxY late (L) budding domains expressed within the matrix proteins of these RNA viruses. As L domains are important for budding and are highly conserved in a wide array ... More
Overcoming mutation-based resistance to antiandrogens with rational drug design.
AuthorsBalbas MD, Evans MJ, Hosfield DJ, Wongvipat J, Arora VK, Watson PA, Chen Y, Greene GL, Shen Y, Sawyers CL,
JournalElife
PubMed ID23580326
'The second-generation antiandrogen enzalutamide was recently approved for patients with castration-resistant prostate cancer. Despite its success, the duration of response is often limited. For previous antiandrogens, one mechanism of resistance is mutation of the androgen receptor (AR). To prospectively identify AR mutations that might confer resistance to enzalutamide, we performed ... More
A plasmid-based reporter system for live cell imaging of dengue virus infected cells.
AuthorsMedin CL, Valois S, Patkar CG, Rothman AL,
Journal
PubMed ID25445884
Cell culture models are used widely to study the effects of dengue virus (DENV) on host cell function. Current methods of identification of cells infected with an unmodified DENV requires fixation and permeablization of cells to allow DENV-specific antibody staining. This method does not permit imaging of viable cells over ... More
HEK-293 cells expressing the cystic fibrosis transmembrane conductance regulator (CFTR): a model for studying regulation of Cl- transport.
AuthorsDomingue JC, Ao M, Sarathy J, George A, Alrefai WA, Nelson DJ, Rao MC,
Journal
PubMed ID25263207
The Human Embryonic Kidney 293 cell line (HEK-293) readily lends itself to genetic manipulation and is a common tool for biologists to overexpress proteins of interest and study their function and molecular regulation. Although these cells have some limitations, such as an inability to form resistive monolayers necessary for studying ... More
Complex determinants in specific members of the mannose receptor family govern collagen endocytosis.
AuthorsJürgensen HJ, Johansson K, Madsen DH, Porse A, Melander MC, Sørensen KR, Nielsen C, Bugge TH, Behrendt N, Engelholm LH,
Journal
PubMed ID24500714
Members of the well-conserved mannose receptor (MR) protein family have been functionally implicated in diverse biological and pathological processes. Importantly, a proposed common function is the internalization of collagen for intracellular degradation occurring during bone development, cancer invasion, and fibrosis protection. This functional relationship is suggested by a common endocytic ... More
Interleukin-1ß Reduces L-type Ca2+ Current through Protein Kinase C? Activation in Mouse Heart.
AuthorsEl Khoury N, Mathieu S, Fiset C,
Journal
PubMed ID24936064
Inflammation is now widely recognized as a key component of heart disease. Patients suffering from arrhythmias and heart failure have increased levels of tumor necrosis factor-a (TNFa) and interleukin-1ß (IL-1ß). Evidence suggests that these cytokines are important mediators of cardiac remodeling; however, their effects on ion channels and arrhythmogenesis remain ... More
Zn2+ dyshomeostasis caused by loss of ATP13A2/PARK9 leads to lysosomal dysfunction and alpha-synuclein accumulation.
AuthorsTsunemi T, Krainc D,
Journal
PubMed ID24334770
Mutations in ATP13A2 (PARK9) cause Kufor-Rakeb syndrome (KRS) characterized by juvenile-onset parkinsonism, pyramidal signs and dementia. PARK9 belongs to type 5 P-type ATPase with its putative function as a cation transporter. Loss of PARK9 leads to lysosomal dysfunction and subsequent a-synuclein (a-Syn) accumulation. However, the mechanistic link between PARK9 and ... More
Centrifugo-magnetophoretic Purification of CD4+ Cells from Whole Blood toward Future HIV/AIDS Point-of-Care Applications.
In medical diagnostics, detection of cells exhibiting specific phenotypes constitutes a paramount challenge. Detection technology must ensure efficient isolation of (often rare) targets while eliminating nontarget background cells. Technologies exist for such investigations, but many require high levels of expertise, expense, and multistep protocols. Increasing automation, miniaturization, and availability of ... More
AuthorsNgo D, Cheng Q, O Connor AE, Deboer KD, Lo CY, Beaulieu E, De Seram M, Hobbs RM, O Bryan MK, Morand EF,
JournalPLoS One
PubMed ID23516608
Spermatogonia stem cell (SSC) self-renewal and differentiation are tightly regulated processes that ensure a continued production of mature sperm throughout male adulthood. In the present study, we investigated the role of glucocorticoid-induced leucine zipper (GILZ) in maintenance of the male germline and spermatogenesis. GILZ was detectable in germ cells of ... More
Surfactant protein-A and toll-like receptor-4 modulate immune functions of preterm baboon lung dendritic cell precursor cells.
AuthorsAwasthi S, Madhusoodhanan R, Wolf R,
JournalCell Immunol
PubMed ID21439559
Lung infections are important risk factors for an increased morbidity and mortality in prematurely-delivered babies. Immaturity of the innate immune components makes them extremely susceptible to infection. Recently, we isolated lung dendritic cell (DC)-precursor cells from preterm fetal baboons. The isolated cells were found to be defective in phagocytosing Escherichia ... More
Three distinct mechanisms generate oxygen free radicals in neurons and contribute to cell death during anoxia and reoxygenation.
AuthorsAbramov AY, Scorziello A, Duchen MR
JournalJ Neurosci
PubMed ID17267568
Ischemia is a major cause of brain damage, and patient management is complicated by the paradoxical injury that results from reoxygenation. We have now explored the generation of reactive oxygen species (ROS) in hippocampal and cortical neurons in culture in response to oxygen and glucose deprivation or metabolic inhibition and ... More
SETDB1 fuels the lung cancer phenotype by modulating epigenome, 3D genome organization and chromatin mechanical properties.
Authors
JournalNucleic Acids Res
PubMed ID35474385
Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics.
Authors
JournalCell
PubMed ID33232692
The SINEB1 element in the long non-coding RNA Malat1 is necessary for TDP-43 proteostasis.
Authors
JournalNucleic Acids Res
PubMed ID31863590
Epithelial colonization by gut dendritic cells promotes their functional diversification.
Authors
JournalImmunity
PubMed ID34910930
Feasibility of using NF1-GRD and AAV for gene replacement therapy in NF1-associated tumors.
AuthorsBai RY, Esposito D, Tam AJ, McCormick F, Riggins GJ, Wade Clapp D, Staedtke V
JournalGene Ther
PubMed ID31127187
'Neurofibromatosis type 1, including the highly aggressive malignant peripheral nerve sheath tumors (MPNSTs), is featured by the loss of functional neurofibromin 1 (NF1) protein resulting from genetic alterations. A major function of NF1 is suppressing Ras activities, which is conveyed by an intrinsic GTPase-activating protein-related domain (GRD). In this study, ... More
TMEM41B Is a Pan-flavivirus Host Factor.
Authors
JournalCell
PubMed ID33338421
Noise-reducing optogenetic negative-feedback gene circuits in human cells.
Authors
JournalNucleic Acids Res
PubMed ID31269201
Harnessing self-labeling enzymes for selective and concurrent A-to-I and C-to-U RNA base editing.
Authors
JournalNucleic Acids Res
PubMed ID34197596
Fabrication of Smart Tantalum Carbide MXene Quantum Dots with Intrinsic Immunomodulatory Properties for Treatment of Allograft Vasculopathy.
Authors
JournalAdv Funct Mater
PubMed ID35153642
Z-form extracellular DNA is a structural component of the bacterial biofilm matrix.
Authors
JournalCell
PubMed ID34735796
Redox-active trace metal-induced release of high mobility group box 1(HMGB1) and inflammatory cytokines in fibroblast-like synovial cells is Toll-like receptor 4 (TLR4) dependent.
AuthorsAlsousi AA, Igwe OJ
JournalBiochim Biophys Acta Mol Basis Dis
PubMed ID30254017
Rheumatoid arthritis (RA) is a chronic autoimmune systemic inflammatory disease that is characterized by synovial inflammation and bone erosion. We have investigated the mechanism(s) by which essential trace metals may initiate and propagate inflammatory phenotypes in synovial fibroblasts. We used HIG-82, rabbit fibroblast-like synovial cells (FLS), as a model system ... More
2-Methoxyestradiol Affects Mitochondrial Biogenesis Pathway and Succinate Dehydrogenase Complex Flavoprotein Subunit A in Osteosarcoma Cancer Cells.
AuthorsGorska-Ponikowska M, Kuban-Jankowska A, Eisler SA, Perricone U, Lo Bosco G, Barone G, Nussberger S,
JournalCancer Genomics Proteomics
PubMed ID29275365
Dysregulation of mitochondrial pathways is implicated in several diseases, including cancer. Notably, mitochondrial respiration and mitochondrial biogenesis are favored in some invasive cancer cells, such as osteosarcoma. Hence, the aim of the current work was to investigate the effects of 2-methoxyestradiol (2-ME), a potent anticancer agent, on the mitochondrial biogenesis ... More
High Affinity Promotes Internalization of Engineered Antibodies Targeting FGFR1.
AuthorsOpalinski L, Szymczyk J, Szczepara M, Kucinska M, Krowarsch D, Zakrzewska M, Otlewski J
JournalInt J Mol Sci
PubMed ID29748524
Fibroblast growth factor receptor 1 (FGFR1) is a plasma membrane protein that transmits signals from the extracellular environment, regulating cell homeostasis and function. Dysregulation of FGFR1 leads to the development of human cancers and noncancerous diseases. Numerous tumors overproduce FGFR1, making this receptor a perspective target for cancer therapies. Antibody-drug ... More
Di-copper metallodrugs promote NCI-60 chemotherapy via singlet oxygen and superoxide production with tandem TA/TA and AT/AT oligonucleotide discrimination.
AuthorsSlator C, Molphy Z, McKee V, Long C, Brown T, Kellett A
JournalNucleic Acids Res
PubMed ID29474633
In order to expand the current repertoire of cancer treatments and to help circumvent limitations associated with resistance, the identification of new metallodrugs with high potency and novel mechanisms of action is of significant importance. Here we present a class of di-copper(II) complex based on the synthetic chemical nuclease [Cu(Phen)2]+ ... More