ReadyProbes™ 细胞活力成像试剂盒(蓝色/绿色)
ReadyProbes™ 细胞活力成像试剂盒(蓝色/绿色)
Invitrogen™

ReadyProbes™ 细胞活力成像试剂盒(蓝色/绿色)

ReadyProbes® 细胞活力成像试剂盒(蓝色/绿色)是一种即用型检测,可快速且轻松地测定细胞活力。只需向 1 mL 细胞生长培养基中加入室温下的稳定 NucBlue® Live了解更多信息
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货号数量
R376091 kit
货号 R37609
价格(CNY)
2,485.00
飞享价
Ends: 31-Dec-2025
3,347.00
共减 862.00 (26%)
Each
添加至购物车
数量:
1 kit
价格(CNY)
2,485.00
飞享价
Ends: 31-Dec-2025
3,347.00
共减 862.00 (26%)
Each
添加至购物车
ReadyProbes® 细胞活力成像试剂盒(蓝色/绿色)是一种即用型检测,可快速且轻松地测定细胞活力。只需向 1 mL 细胞生长培养基中加入室温下的稳定 NucBlue® Live 试剂 (Hoechst 33342) 和 NucGreen® Dead 试剂各2滴,然后即可通过计数总细胞与死细胞来确定细胞活力。NucBlue® Live 试剂可对所有细胞的细胞核进行染色,并可使用标准 DAPI 滤光片进行检测。NucGreen® Dead 试剂仅对细胞膜完整性受损细胞的细胞核进行染色,并能使用标准 FITC/GFP(绿色)滤光片组进行检测。本试剂盒适用于荧光显微镜、荧光微孔板读数仪和流式细胞分析。

NucBlue® Live 试剂: 可对所有细胞的细胞核进行染色;使用标准 DAPI 滤光片进行检测(最大激发/发射波长:360/460 nm)
NucGreen® Dead 试剂: 仅对细胞膜受损的死细胞的细胞核进行染色;使用标准 FITC/GFP(绿色)滤光片组进行检测(最大激发/发射波长:504/523 nm)
请参阅其他 ReadyProbes® 细胞染色试剂
进一步了解其他细胞活力测定试剂盒

使用建议
•NucBlue® Live 和 NucGreen® Dead 试剂可直接加入完全生长培养基的细胞或兼容的缓冲液中。
• 在大多数情况下,2滴/mL 以及5至30分钟的孵育时间将使细胞核染上明亮颜色;然而,可能需要针对一些细胞类型、条件和应用进行优化。在此种情况下,只需简单地加入更多或更少的液滴,直至获得较佳染色强度。
仅供科研使用。不可用于诊断程序。
规格
细胞类型哺乳动物细胞、真核细胞
描述ReadyProbes 细胞活力成像试剂盒,蓝色/绿色
检测方法荧光
染料类型3 x 2.5 mL NucBlue™ Live
产品规格滴瓶
数量1 kit
运输条件室温
颜色蓝色、绿色
发射360、504 nm
Excitation Wavelength Range460、523 nm
适用于(应用)活力测定试剂盒
适用于(设备)荧光显微镜
产品线ReadyProbes
产品类型细胞活力成像试剂盒
Unit SizeEach
内容与储存
在 2° 至 8°C 或室温下储存。

常见问题解答 (FAQ)

我在 Countess II FL全自动细胞计数仪上能进行哪种荧光活性实验?

我们已经确认可在Countess II FL全自动细胞计数仪上使用下列试剂盒:

•LIVE/DEAD Viabilty/Cytoxicity试剂盒(货号L3224)含有钙黄绿素AM和溴乙啡锭二聚体
•ReadyProbes Cell Viability Imaging试剂盒,蓝/绿(货号R37609)
•ReadyProbes Cell Viability Imaging试剂盒,蓝/红(货号R37610)含有NucBlue Live/NucGreen Dead和NucBlue Live/propidium iodide

见此应用说明(https://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/Laboratory%20Instruments/Files/0415/CO014723-Countess-II-FL-Viability-Appnote_FHR.pdf)中的详情。

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What fluorescent viability assays can I use on the Countess II FL automated cell counter?

We have validated the following kits for use on the Countess II FL Automated Cell Counter:

LIVE/DEAD Viabilty/Cytoxicity Kit (Cat. No. L3224) containing calcein AM and ethidium homodimer-1
ReadyProbes Cell Viability Imaging Kit, Blue/Green (Cat. No. R37609)
ReadyProbes Cell Viability Imaging Kit, Blue/Red (Cat. No. R37610) containing NucBlue Live/NucGreen Dead and NucBlue Live/propidium iodide
See this Application Note for details - https://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/Laboratory%20Instruments/Files/0415/CO014723-Countess-II-FL-Viability-Appnote_FHR.pdf.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between NucBlue Live reagents stain and NucGreen Dead reagents stain?

The NucBlue Live reagent stains the nuclei of all cells, while NucGreen Dead reagent stains only the nuclei of dead cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.