ReadyProbes™ 2.5% Normal Horse Serum (1X) - FAQs

查看更多产品信息 ReadyProbes™ 2.5% Normal Horse Serum (1X) - FAQs (R37625)

4 个常见问题解答

What is the volume for one drop from the ReadyProbes dropper bottles?

The volume for one drop from the ReadyProbes dropper bottles is approximately 41 µL.

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Can I use ReadyProbes 2.5% Normal Horse Serum (1X) as an antibody diluent for both primary and secondary antibodies?

Yes, ReadyProbes 2.5% Normal Horse Serum (1X) can be used as an antibody diluent for both primary and secondary antibodies, except for HRP-conjugated antibodies. The sodium azide added as a preservative is an inhibitor of horseradish peroxidase.

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How many drops of ReadyProbes 2.5% Normal Horse Serum (1X) should I add to a sample?

Add a sufficient number of drops of ReadyProbes 2.5% Normal Horse Serum (1X) to completely cover the sample.

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When would I use goat, horse, or chicken serum for blocking versus FBS or BSA?

For blocking, you should use a serum of the same or closely related species of the secondary antibody being used. If using a secondary antibody that was raised in goat or sheep, use goat serum (Cat. No. R37624 or 31873) for blocking; if using a secondary antibody raised in donkey or horse, use horse serum (Cat. No. R37625 or 31874) for blocking; if using a secondary antibody raised in chicken, use chicken serum (Cat. No. R37626) for blocking. FBS and BSA are alternatives when a specific species of serum is not available.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.