ReadyProbes™ 亲和素/生物素封闭溶液 (1X)

ReadyProbes Avidin/Biotin Blocking Solution blocks endogenous biotin, biotin receptors, and avidin binding sites present in many types of tissue, reducing了解更多信息

货号	数量
R37627	1 个试剂盒

货号 R37627

价格（CNY)

1,409.00

Ends: 31-Dec-2025

1,898.00

共减 489.00 (26%)

添加至购物车

1 个试剂盒

价格（CNY)

1,409.00

Ends: 31-Dec-2025

1,898.00

共减 489.00 (26%)

添加至购物车

ReadyProbes Avidin/Biotin Blocking Solution blocks endogenous biotin, biotin receptors, and avidin binding sites present in many types of tissue, reducing background signal in immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and in situ hybridization (ISH) procedures. This two-component solution (an avidin-blocking solution and a biotin-blocking solution) is provided in a ready-to-use format—just add a few drops of the avidin component and incubate for 15 minutes, then wash and repeat with the biotin component. ReadyProbes Avidin/Biotin Blocking Solution is used after the blocking of cells/tissue with standard non-specific blocking solution (e.g., diluted serum), but before the primary antibody step.

仅供科研使用。不可用于诊断程序。

产品类型亲和素/生物素封闭溶液

运输条件经批准可在室温下或置于湿冰上运输

产品线ReadyProbes™

数量1 个试剂盒

Unit SizeEach

内容与储存

由两个透明 LDPE 滴瓶分别盛装 18 mL 亲和素（组分 A）和生物素（组分 B）即用型 (1X) 溶液的试剂盒。

在 2–8°C 下储存。

根据指示储存时，产品可以在收到后 6 个月内使用。

常见问题解答 (FAQ)

With the ReadyProbes Avidin/Biotin Blocking Solution (1X), why is there a step to add biotin to the sample?

The first step is to bind all endogenous biotinylated entities with an unlabeled avidin. The free D-biotin is then added to bind to all biotin binding sites on this added avidin. The free biotin will be washed away before any other labels are added and all biotin binding sites will be occupied on the unlabeled avidin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am labeling cells with a biotinylated primary antibody, followed by an avidin dye conjugate, but I'm getting non-specific mitochondrial labeling, even with an avidin-only control. Why is this?

Mitochondria have endogenous biotin, which is being labeled by the streptavidin. You will need to block the endogenous biotin first, after you fix and permeabilize, but before you block for your primary antibody.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is ReadyProbes Avidin/Biotin Blocking Solution (1X) different from other endogenous biotin blocking kits?

The only difference between the ReadyProbes Avidin/Biotin Blocking Solution (1X) and other endogenous biotin blocking kits is the ease of use. In the ReadyProbes Avidin/Biotin Blocking Solution (1X), we provide the two components already pre-mixed and ready to use.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.