ReadyProbes™ Streptavidin/Biotin Blocking Solution (1X) - FAQs

查看更多产品信息 ReadyProbes™ Streptavidin/Biotin Blocking Solution (1X) - FAQs (R37628)

4 个常见问题解答

What is the volume for one drop from the ReadyProbes dropper bottles?

The volume for one drop from the ReadyProbes dropper bottles is approximately 41 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

In the ReadyProbes Streptavidin/Biotin Blocking Solution (1X) protocol, why is there a step to add biotin to the sample?

The first step in this kit is to bind all endogenous biotinylated entities with an unlabeled streptavidin. The free D-biotin is added to bind to all biotin binding sites on the added streptavidin. The free biotin will be washed away before any other labels are added and all biotin binding sites will be occupied on the unlabeled streptavidin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am labeling cells with a biotinylated primary antibody, followed by a streptavidin dye conjugate, but I'm getting non-specific mitochondrial labeling, even with a streptavidin-only control. Why?

Mitochondria have endogenous biotin, which is being labeled by the streptavidin. You will need to block the endogenous biotin first, after you fix and permeabilize, but before you block for your primary antibody.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is ReadyProbes Streptavidin/Biotin Blocking Solution (1X) different from other endogenous biotin blocking kits?

The only difference between the ReadyProbes Streptavidin/Biotin Blocking Solution (1X) and other endogenous biotin blocking kits is the use of streptavidin (as other kits use avidin) and the ease of use. In the ReadyProbes Streptavidin/Biotin Blocking Solution (1X), we provide the two components already pre-mixed and ready to use.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.