A biosensor for inorganic phosphate using a rhodamine-labeled phosphate binding protein.
AuthorsOkoh MP, Hunter JL, Corrie JE, Webb MR
JournalBiochemistry
PubMed ID17144669
'A novel biosensor for inorganic phosphate (Pi) has been developed based on the phosphate binding protein of Escherichia coli. Two cysteine mutations were introduced and labeled with 6-iodoacetamidotetramethylrhodamine. When physically close to each other and correctly oriented, two rhodamine dyes interact to form a noncovalent dimer. In this state, they ... More
Dependence of fluorescence intensity on the spectral overlap between fluorophores and plasmon resonant single silver nanoparticles.
AuthorsChen Y, Munechika K, Ginger DS
JournalNano Lett
PubMed ID17315937
'We investigate the fluorescence from dyes coupled to individual DNA-functionalized metal nanoparticles. We use single-particle darkfield scattering and fluorescence microscopy to correlate the fluorescence intensity of the dyes with the localized surface plasmon resonance (LSPR) spectra of the individual metal nanoparticles to which they are attached. For each of three ... More
Filling the green gap of the major light-harvesting chlorophyll a/b complex by covalent attachment of Rhodamine Red.
AuthorsGundlach K, Werwie M, Wiegand S, Paulsen H,
JournalBiochim Biophys Acta
PubMed ID19619502
'The major light-harvesting chlorophyll a/b complex (LHCII) greatly enhances the efficiency of photosynthesis in green plants. Recombinant LHCII can be assembled in vitro from its denatured, bacterially expressed apoprotein and plant pigments. This makes it an interesting candidate for biomimetic light-harvesting in photovoltaic applications. Due to its almost 20 pigments ... More
Thiol-reactive dyes for fluorescence labeling of proteomic samples.
AuthorsTyagarajan K, Pretzer E, Wiktorowicz JE
JournalElectrophoresis
PubMed ID12874870
Covalent derivatization of proteins with fluorescent dyes prior to separation is increasingly used in proteomic research. This paper examines the properties of several commercially available iodoacetamide and maleimide dyes and discusses the conditions and caveats for their use in labeling of proteomic samples. The iodoacetamide dyes BODIPY TMR cadaverine IA ... More