Fluorescent rhodol derivatives: versatile, photostable labels and tracers.
AuthorsWhitaker JE, Haugland RP, Ryan D, Hewitt PC, Haugland RP, Prendergast FG
JournalAnal Biochem
PubMed ID1481981
A series of chemically reactive, fluorescent rhodol derivatives was prepared and evaluated. Reactive functional groups included activated esters, amines, haloacetamides, fixable hydrazide derivatives, acrylamides, and photoaffinity reagents. Depending on the choice of substituents, absorption maxima of the dyes varied from 490 to 550 nm with extinction coefficients that were generally ... More
Stains, labels and detection strategies for nucleic acids assays.
AuthorsKricka LJ
JournalAnn Clin Biochem
PubMed ID11928759
Selected developments and trends in stains, labels and strategies for detecting and measuring nucleic acids (DNA, RNA) and related molecules [e.g. oligo(deoxy)nucleotides, nucleic acid fragments and polymerase chain reaction products] are surveyed based on the literature in the final decade of the 20th century (1991-2000). During this period, important families ... More