Evolution of peptides that modulate the spectral qualities of bound, small-molecule fluorophores.
AuthorsRozinov MN, Nolan GP
JournalChem Biol
PubMed ID9862799
'BACKGROUND: Fluorophore dyes are used extensively in biomedical research to sensitively assay cellular constituents and physiology. We have created, as proof of principle, fluorophore dye binding peptides that could have applications in fluorescent dye-based approaches in vitro and in vivo. RESULTS: A panel of Texas red, Rhodamine red, Oregon green ... More
Spectroscopic investigation of a FRET molecular beacon containing two fluorophores for probing DNA/RNA sequences.
AuthorsJockusch S, Martí AA, Turro NJ, Li Z, Li X, Ju J, Stevens N, Akins DL
JournalPhotochem Photobiol Sci
PubMed ID16685327
'We report the design, synthesis, and characterization of a molecular beacon (MB) consisting of two fluorescent dyes (Alexa 488 and RedX) for DNA and RNA analysis. In the absence of the target DNA or RNA the MB is in its stem-closed form and shows efficient energy transfer from the donor ... More
Delivery of oligonucleotides into mammalian cells by anionic peptides: comparison between monomeric and dimeric peptides.
AuthorsFreulon I, Roche AC, Monsigny M, Mayer R
JournalBiochem J
PubMed ID11237872
'The use of antisense oligonucleotides as putative therapeutic agents is limited by their poor delivery into the cytosol and/or the nucleus because they are not able to efficiently cross lipid bilayers. To circumvent this pitfall, anionic amphipathic peptides derived from the influenza virus fusogenic peptide have been used to destabilize ... More
Chemistry of sulforhodamine--amine conjugates.
AuthorsCorrie JE, Davis CT, Eccleston JF
JournalBioconjug Chem
PubMed ID11312679
Commercially-available sulforhodamine sulfonyl chlorides contain two isomeric monosulfonyl chlorides. Conjugates of these isomers with amines have different properties because the sulfonamide formed from one isomer can undergo ring-closure to a colorless sultam. This chemistry has been examined for a model conjugate with methylamine and for a bioconjugate with 2'(3')-O-[N-(2-aminoethyl)carbamoyl]ATP. The ... More
Imaging of the fluorescence spectrum of a single fluorescent molecule by prism-based spectroscopy.
AuthorsSuzuki Y, Tani T, Sutoh K, Kamimura S
JournalFEBS Lett
PubMed ID11852087
We have devised a novel method to visualize the fluorescence spectrum of a single fluorescent molecule using prism-based spectroscopy. Equipping a total internal reflection microscope with a newly designed wedge prism, we obtained a spectral image of a single rhodamine red molecule attached to an essential light chain of myosin. ... More
Rhodamine-labeled 2beta-carbomethoxy-3beta-(3,4-dichlorophenyl)tropane analogues as high-affinity fluorescent probes for the dopamine transporter.
AuthorsCha JH, Zou MF, Adkins EM, Rasmussen SG, Loland CJ, Schoenenberger B, Gether U, Newman AH
JournalJ Med Chem
PubMed ID16302792
Novel fluorescent ligands were synthesized to identify a high-affinity probe that would enable visualization of the dopamine transporter (DAT) in living cells. Fluorescent tags were extended from the N- or 2-position of 2beta-carbomethoxy-3beta-(3,4-dichlorophenyl)tropane, using an ethylamino linker. The resulting 2-substituted (5) and N-substituted (9) rhodamine-labeled ligands provided the highest DAT ... More
Fluorescent histochemical techniques for analysis of intracellular signaling.
AuthorsOksvold MP, Skarpen E, Widerberg J, Huitfeldt HS
JournalJ Histochem Cytochem
PubMed ID11850432
Intracellular signaling relies on the orchestrated cooperation of signaling proteins and modules, their intracellular localization, and membrane trafficking. Recently, a repertoire of fluorescence-based techniques, which significantly increases our potential for detailed studies of the involved mechanisms, has been introduced. Microscopic techniques with increased resolution have been combined with improved techniques ... More
Simultaneous measurement of one- and two-photon excited fluorescence from a single sample: a detection method for oligonucleotides.
AuthorsAlexander T, Tran CD
JournalAppl Opt
PubMed ID12003221
A new method has been developed that is based on the use of a single-excitation wavelength from a cw laser to excite simultaneously one-photon and two-photon fluorescence (TPF). Fluorescence bands of a sample containing two oligonucleotides, one labeled with a one-photon fluorescence dye and the other with a TPF dye, ... More
The lateral diffusion of selectively aggregated peptides in giant unilamellar vesicles.
AuthorsLee CC, Revington M, Dunn SD, Petersen NO
JournalBiophys J
PubMed ID12609877
We have systematically investigated the effect of aggregation of a transmembrane peptide on its diffusion in dimyristoylphosphatidylcholine and in palmitoyloleoylphosphatidylcholine model membranes. The hydrophobic segment of the b subunit from E. coli F(1)F(0)-ATP synthase was modified with a histidine tag at the carbonyl terminus and was aggregated selectively by using ... More
Transcription of giant DNA complexed with cationic nanoparticles as a simple model of chromatin.
AuthorsZinchenko AA, Luckel F, Yoshikawa K
JournalBiophys J
PubMed ID17142281
We prepared complexes of giant double-stranded DNA with cationic nanoparticles of 10-40 nm in diameter as an artificial model of chromatin and characterized the properties of changes in their higher-order conformation. We measured the changes in transcriptional activity that accompanied the DNA conformational transitions. Complete inhibition was found at excess ... More
Detection of receptor clustering by flow cytometric fluorescence anisotropy measurements.
AuthorsBene L, Fulwyler MJ, Damjanovich S
JournalCytometry
PubMed ID10918280
BACKGROUND: Perrin equation suggests an alternative way for the accurate energy transfer determination on a cell-by-cell basis by measuring polarized donor intensities in a conventional flow cytometer. METHODS: The relationship between energy transfer and fluorescence anisotropy of the donor was investigated by flow cytometric generation of Perrin-lifetime plots of fluorescent ... More
Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture.
AuthorsKrahn KN, Bouten CV, van Tuijl S, van Zandvoort MA, Merkx M
JournalAnal Biochem
PubMed ID16476406
Visualization of the formation and orientation of collagen fibers in tissue engineering experiments is crucial for understanding the factors that determine the mechanical properties of tissues. In this study, collagen-specific fluorescent probes were developed using a new approach that takes advantage of the inherent specificity of collagen binding protein domains ... More
Lipoprotein receptors in extraembryonic tissues of the chicken.
AuthorsHermann M, Mahon MG, Lindstedt KA, Nimpf J, Schneider WJ
JournalJ Biol Chem
PubMed ID10747878
Yolk is the major source of nutrients for the developing chicken embryo, but molecular details of the delivery mechanisms are largely unknown. During oogenesis in the chicken, the main yolk components vitellogenin and very low density lipoprotein (VLDL) are taken up into the oocytes via a member of the low ... More
Texas Res-X and rhodamine Red-X, new derivatives of sulforhodamine 101 and lissamine rhodamine B with improved labeling and fluorescence properties.
AuthorsLefevre C, Kang HC, Haugland RP, Malekzadeh N, Arttamangkul S, Haugland RP
JournalBioconjug Chem
PubMed ID8853462
Texas Red sulfonyl chloride (TR-SC) and Lissamine rhodamine B sulfonyl chloride (LRB-SC) are popular dyes often used to prepare red fluorescent conjugates that are useful second labels in combination with fluorescein. Unfortunately, being sulfonyl chloride derivatives, both are unstable to moisture during storage and prone to hydrolysis in the conjugation ... More
Luminescent quantum dots fluorescence resonance energy transfer-based probes for enzymatic activity and enzyme inhibitors.
AuthorsShi L, Rosenzweig N, Rosenzweig Z
JournalAnal Chem
PubMed ID17194141
The paper describes the development and characterization of analytical properties of quantum dot-based probes for enzymatic activity and for screening enzyme inhibitors. The luminescent probes are based on fluorescence resonance energy transfer (FRET) between luminescent quantum dots that serve as donors and rhodamine acceptors that are immobilized to the surface ... More