Imaging hair cell transduction at the speed of sound: dynamic behavior of mammalian stereocilia.
AuthorsFridberger A, Tomo I, Ulfendahl M, Boutet de Monvel J
JournalProc Natl Acad Sci U S A
PubMed ID16446441
'The cochlea contains two types of sensory cells, the inner and outer hair cells. Sound-evoked deflection of outer hair cell stereocilia leads to fast force production that will enhance auditory sensitivity up to 1,000-fold. In contrast, inner hair cells are thought to have a purely receptive function. Deflection of their ... More
Near infrared two-photon excitation cross-sections of voltage-sensitive dyes.
AuthorsFisher JA, Salzberg BM, Yodh AG
JournalJ Neurosci Methods
PubMed ID16129493
'Microscopy based on voltage-sensitive dyes has proven effective for revealing spatio-temporal patterns of neuronal activity in vivo and in vitro. Two-photon microscopy using voltage-sensitive dyes offers the possibility of wide-field visualization of membrane potential on sub-cellular length scales, hundreds of microns below the tissue surface. Very little information is available, ... More
Optical current source density analysis in hippocampal organotypic culture shows that spreading depression occurs with uniquely reversing currents.
AuthorsKunkler PE, Hulse RE, Schmitt MW, Nicholson C, Kraig RP
JournalJ Neurosci
PubMed ID15829647
'Spreading depression (SD) involves current flow through principal neurons, but the pattern of current flow over the expanse of susceptible tissues or individual principal neurons remains undefined. Accordingly, tissue and single cell maps made from digital imaging of voltage-sensitive dye changes in hippocampal organotypic cultures undergoing SD were processed via ... More
Novel naphthylstyryl-pyridium potentiometric dyes offer advantages for neural network analysis.
AuthorsObaid AL, Loew LM, Wuskell JP, Salzberg BM
JournalJ Neurosci Methods
PubMed ID15003384
'The submucous plexus of the guinea pig intestine is a quasi-two-dimensional mammalian neural network that is particularly amenable to study using multiple site optical recording of transmembrane voltage (MSORTV) [Biol. Bull. 183 (1992) 344; J. Neurosci. 19 (1999) 3073]. For several years the potentiometric dye of choice for monitoring the ... More
In vivo observations of pre- and postsynaptic changes during the transition from multiple to single innervation at developing neuromuscular junctions.
AuthorsBalice-Gordon RJ, Lichtman JW
JournalJ Neurosci
PubMed ID8426240
'Synaptic rearrangements in developing muscle were studied by visualizing individual neuromuscular junctions in the sternomastoid muscle of living neonatal mice as they underwent the transition from multiple to single innervation. Vital staining of ACh receptors (AChRs) with rhodamine-conjugated alpha-bungarotoxin showed that while junctions were still multiply innervated (usually by two ... More
Visual stimuli induce waves of electrical activity in turtle cortex.
AuthorsPrechtl JC, Cohen LB, Pesaran B, Mitra PP, Kleinfeld D
JournalProc Natl Acad Sci U S A
PubMed ID9207142
'The computations involved in the processing of a visual scene invariably involve the interactions among neurons throughout all of visual cortex. One hypothesis is that the timing of neuronal activity, as well as the amplitude of activity, provides a means to encode features of objects. The experimental data from studies ... More
Vital staining of the hearing organ: visualization of cellular structure with confocal microscopy.
AuthorsFlock A, Scarfone E, Ulfendahl M
JournalNeuroscience
PubMed ID9466411
'Cells inside the intact organ of Corti were labelled with fluorescent probes reflecting various aspects of structure and function. The dyes were introduced into the perilymphatic space by perfusion of the scala tympani of the temporal bone from the guinea-pig maintained in isolation. The dyes were able to diffuse through ... More
A new neurophysiological/neuropathological ex vivo model localizes the origin of glioma-associated epileptogenesis in the invasion area.
AuthorsSenner V, Köhling R, Püttmann-Cyrus S, Straub H, Paulus W, Speckmann EJ
JournalActa Neuropathol (Berl)
PubMed ID13680280
'Seizures commonly occur in glioma patients, but their pathogenesis is poorly understood, in part due to a lack of valid and versatile experimental models. We have established a new model that enables comprehensive neuropathological and neurophysiological analysis on identical tissue preparations. Rat C6 glioma cells stably transfected with a green ... More
Imaging the living inner ear using intravital confocal microscopy.
AuthorsTomo I, Le Calvez S, Maier H, Boutet de Monvel J, Fridberger A, Ulfendahl M
JournalNeuroimage
PubMed ID17382563
'Confocal laser scanning microscopy permits detailed visualization of structures deep within thick fluorescently labeled specimen. This makes it possible to investigate living cells inside intact tissue without prior chemical sample fixation and sectioning. Isolated guinea pig temporal bones have previously been used for confocal experiments in vitro, but tissue deterioration ... More
Comparison of fluorescent voltage-sensitive dyes for multisite optical recording in hamster cerebral cortex by measurement of bicuculline-induced epileptiform events.
AuthorsdeBeer Zeiger J
JournalNeuroimage
PubMed ID9345545
'Two fluorescent voltage-sensitive dyes, RH795 and DI-2-ANEPPQ, were compared for in vivo multisite optical recording from the gustatory insular cortex of the golden Syrian hamster, the first reported use of DI-2-ANEPPQ in a mammalian brain preparation. The exposed cortex of the anesthetized hamster was stained with a 500 microM solution ... More
Deblurring of 3-dimensional patterns of evoked rat cerebellar cortical activity: a study using voltage-sensitive dyes and optical sectioning.
AuthorsYae H, Elias SA, Ebner TJ
JournalJ Neurosci Methods
PubMed ID1501504
'One of the benefits of imaging neuronal activity is the capability of resolving spatial patterns in the x-y plane. With optical sectioning microscopy, the 3-dimensional (3-D) structure may also be studied without physical deformation by serially moving the focal plane of the microscope through the volume of interest along the ... More
Temporal binding via cortical coincidence detection of specific and nonspecific thalamocortical inputs: a voltage-dependent dye-imaging study in mouse brain slices.
AuthorsLlinas RR, Leznik E, Urbano FJ
JournalProc Natl Acad Sci U S A
PubMed ID11773628
'Voltage-sensitive dye imaging of mouse thalamocortical slices demonstrated that electrical stimulation of the centrolateral intralaminar thalamic nucleus (CL) resulted in the specific activation of thalamic reticular nucleus, striatum/putamen, and cortical layers 5, 6, and 1. By contrast, ventrobasal (VB) thalamic stimulation, while activating the reticular and basal ganglia nuclei, also ... More
Origin of the in vivo rat piriform cortex activity recorded with voltage-sensitive dyes: comparison of the optical signals and the field potentials.
AuthorsLitaudon P, Cattarelli M
JournalBrain Res
PubMed ID1467937
'The comparison of optical recordings and evoked field potentials recorded on the rat piriform cortex pointed out that both signals were strongly correlated. As the field potentials, the two waves of the optical signals originated from the mono- (direct olfactory bulb afferents) and polysynaptic (intrinsic association fibers) excitatory postsynaptic potentials. ... More
Localization of the site of Ca2+ release at the level of a single sarcomere in skeletal muscle fibres.
'The development of mechanical force in skeletal muscle fibres is brought about by rapid increases in the intracellular calcium concentration (Ca2+ transients) which can be detected by optical methods. Local stimulation experiments and ultrastructural evidence suggest that, at a microscopic level, these Ca2+ transients are generated by the release of ... More
Optical imaging of neuronal activity.
AuthorsGrinvald A, Frostig RD, Lieke E, Hildesheim R
JournalPhysiol Rev
PubMed ID3054949
Voltage-sensitive dyes and functional activity in the olfactory pathway.
AuthorsCinelli AR, Kauer JS
JournalAnnu Rev Neurosci
PubMed ID1575446
Voltage-sensitive dye imaging: Technique review and models.
AuthorsChemla S, Chavane F,
JournalJ Physiol Paris
PubMed ID19909809
In this review, we present the voltage-sensitive dye imaging (VSDI) method. The possibility offered for in vivo (and in vitro) brain imaging is unprecedented in terms of spatial and temporal resolution. However, the unresolved multi-component origin of the optical signal encourages us to perform a detailed analysis of the method ... More
In vivo fluorescence microscopy of neuronal activity in three dimensions by use of voltage-sensitive dyes.
AuthorsFisher JA, Civillico EF, Contreras D, Yodh AG
JournalOpt Lett
PubMed ID14719664
We report in vivo imaging of neuronal electrical activity from superficial layers of the mouse barrel cortex. The measurements have approximately 16-microm spatial and 3-ms temporal resolution and reach depths of 150 microm below the cortical surface. The depth-dependent differential-fluorescence optical sections of activity are consistent with known cortical architecture ... More
Optical imaging of interaural time difference representation in rat auditory cortex.
AuthorsTsytsarev V, Fukuyama H, Pope D, Pumbo E, Kimura M,
JournalFront Neuroengineering
PubMed ID19277218
We used in vivo voltage-sensitive dye optical imaging to examine the cortical representation of interaural time difference (ITD), which is believed to be involved in sound source localization. We found that acoustic stimuli with dissimilar ITD activate various localized domains in the auditory cortex. The main loci of the activation ... More
Sound-induced length changes in outer hair cell stereocilia.
AuthorsHakizimana P, Brownell WE, Jacob S, Fridberger A,
JournalNat Commun
PubMed ID23033070
Hearing relies on mechanical stimulation of stereocilia bundles on the sensory cells of the inner ear. When sound hits the ear, each stereocilium pivots about a neck-like taper near their base. More than three decades of research have established that sideways deflection of stereocilia is essential for converting mechanical stimuli ... More
In vitro functional imaging in brain slices using fast voltage-sensitive dye imaging combined with whole-cell patch recording.
AuthorsCarlson GC, Coulter DA,
JournalNat Protoc
PubMed ID18274527
In many brain areas, circuit connectivity is segregated into specific lamina or glomerula. Functional imaging in these anatomically discrete areas is particularly useful in characterizing circuit properties. Voltage-sensitive dye (VSD) imaging directly assays the spatiotemporal dynamics of neuronal activity, including the functional connectivity of the neurons involved. In spatially segregated ... More
Anatomical and functional imaging of neurons using 2-photon laser scanning microscopy.
AuthorsDenk W, Delaney KR, Gelperin A, Kleinfeld D, Strowbridge BW, Tank DW, Yuste R
JournalJ Neurosci Methods
PubMed ID7869748
Light scattering by brain tissue and phototoxicity are major obstacles to the use of high-resolution optical imaging and photo-activation ('uncaging') of bioactive compounds from inactive ('caged') precursors in intact and semi-intact nervous systems. Optical methods based on 2-photon excitation promise to reduce these obstacles (Denk, 1994; Denk et al., 1990, ... More
Optical recordings of the cortical response to whisker stimulation before and after the addition of an epileptogenic agent.
AuthorsLondon JA, Cohen LB, Wu JY
JournalJ Neurosci
PubMed ID2723769
Optical recording methods using voltage-sensitive dyes were used to monitor activity in rat somatosensory cortex. We measured evoked signals in response to whisker stimulation before (control) and after the addition of the epileptogenic agent, bicuculline, and also detected spontaneous interictal events that occurred after bicuculline. Bicuculline led to an increase ... More
Cortical feedback depolarization waves: a mechanism of top-down influence on early visual areas.
AuthorsRoland PE, Hanazawa A, Undeman C, Eriksson D, Tompa T, Nakamura H, Valentiniene S, Ahmed B
JournalProc Natl Acad Sci U S A
PubMed ID16891418
Despite the lack of direct evidence, it is generally believed that top-down signals are mediated by the abundant feedback connections from higher- to lower-order sensory areas. Here we provide direct evidence for a top-down mechanism. We stained the visual cortex of the ferret with a voltage-sensitive dye and presented a ... More
Fluorescent styryl dyes as probes for Na,K-ATPase reaction mechanism: significance of the charge of the hydrophilic moiety of RH dyes.
AuthorsFedosova NU, Cornelius F, Klodos I
JournalBiochemistry
PubMed ID8527456
The fluorescence responses of a series of potential-sensitive styryl-based dyes (either zwitterionic RH160, RH421, di-4-ANEPPS, or positively charged RH795, RH414, RH461) to phosphorylation of Na,K-ATPase from ATP or inorganic phosphate, and ouabain binding to phospho- or dephosphoforms, have been characterized and compared in broken membrane preparations of the enzyme. Zwitterionic ... More
Cortical point-spread function and long-range lateral interactions revealed by real-time optical imaging of macaque monkey primary visual cortex.
AuthorsGrinvald A, Lieke EE, Frostig RD, Hildesheim R
JournalJ Neurosci
PubMed ID8182427
Processing of retinal images is carried out in the myriad dendritic arborizations of cortical neurons. Such processing involves complex dendritic integration of numerous inputs, and the subsequent output is transmitted to multiple targets by extensive axonal arbors. Thus far, details of this intricate processing remained unexaminable. This report describes the ... More
In vivo tracing of pathways and spatio-temporal activity patterns in rat visual cortex using voltage sensitive dyes.
AuthorsOrbach HS, Van Essen DC
JournalExp Brain Res
PubMed ID8359253
We monitored optical signals from cortex stained with a voltage sensitive dye to study activity evoked by intracortical electrical stimulation. The objectives were to study the spatial and temporal spread of activity from intrinsic connections near the stimulating electrode and to develop a new technique to study extrinsic projections from ... More
Formation of specific afferent connections in organotypic slice cultures from rat visual cortex cocultured with lateral geniculate nucleus.
AuthorsBolz J, Novak N, Staiger V
JournalJ Neurosci
PubMed ID1494945
The development of the cerebral cortex involves the specification of intrinsic circuitry and extrinsic connections, the pattern of inputs and outputs. To investigate the development of a major afferent input to the cortex, we studied the formation of thalamocortical connections in an organotypic culture system. Slices from the lateral thalamus ... More