Mediator is a transducer of Wnt/beta -catenin signaling.
AuthorsKim S, Xu X, Hecht A, Boyer TG,
JournalJ Biol Chem
PubMed ID16565090
'Signal transduction within the canonical Wnt/b-catenin pathway drives development and carcinogenesis through programmed or un-programmed changes in gene transcription. While the upstream events linked to signal-induced activation of b-catenin in the cytoplasm have been deciphered in considerable detail, much less is known regarding the mechanism by which b-catenin stimulates target ... More
Hsp27 Enhances Recovery of Splicing as well as Rephosphorylation of SRp38 after Heat Shock.
Monitoring Editor: Greg Matera A heat stress causes a rapid inhibition of splicing. Exogenous expression of Hsp27 did not prevent that inhibition but enhanced the recovery of splicing afterward. Another small heat shock protein, alphaB-crystallin, had no effect. Hsp27, but not alphaB-crystallin, also hastened rephosphorylation of SRp38 - dephosphorylated a ... More
Tumor inhibition by genomically integrated inducible RNAi-cassettes.
AuthorsKappel S, Matthess Y, Zimmer B, Kaufmann M, Strebhardt K,
JournalNucleic Acids Res
PubMed ID16945954
RNA interference (RNAi) has emerged as a powerful tool to induce loss-of-function phenotypes by post-transcriptional silencing of gene expression. In this study we wondered whether inducible RNAi-cassettes integrated into cellular DNA possess the power to trigger neoplastic growth. For this purpose inducible RNAi vectors containing tetracycline (Tet)-responsive derivatives of the ... More
Increased production of reactive oxygen species in hyperglycemic conditions requires dynamic change of mitochondrial morphology.
AuthorsYu T, Robotham JL, Yoon Y,
JournalProc Natl Acad Sci U S A
PubMed ID16477035
Increased production of mitochondrial reactive oxygen species (ROS) by hyperglycemia is recognized as a major cause of the clinical complications associated with diabetes and obesity [Brownlee, M. (2001) Nature 414, 813-820]. We observed that dynamic changes in mitochondrial morphology are associated with high glucose-induced overproduction of ROS. Mitochondria undergo rapid ... More