Intracellular delivery mediated by an ethosomal carrier.
AuthorsTouitou E, Godin B, Dayan N, Weiss C, Piliponsky A, Levi-Schaffer F
JournalBiomaterials
PubMed ID11575480
'The goal of this work was to investigate the efficiency of transcellular delivery into Swiss albino mice 3T3 fibroblasts of molecules with various physico-chemical characteristics from ethosomes, phospholipid vesicular carriers containing ethanol. The probes chosen were: 4-(4-diethylamino) styryl-N-methylpyridinium iodide (D-289), rhodamine red dihexadecanoylglycerophosphoethanolamine (RR) and fluorescent phosphatidylcholine (PC*). The penetration ... More
A lipid-specific toxin reveals heterogeneity of sphingomyelin-containing membranes.
AuthorsIshitsuka R, Yamaji-Hasegawa A, Makino A, Hirabayashi Y, Kobayashi T
JournalBiophys J
PubMed ID14695271
Little is known about the heterogenous organization of lipids in biological membranes. Sphingomyelin (SM) is a major plasma membrane lipid that forms lipid domains together with cholesterol and glycolipids. Using SM-specific toxin, lysenin, we showed that in cultured epithelial cells the accessibility of the toxin to SM is different between ... More