StemPro™ Human Adipose-Derived Stem Cell Kit - FAQs

间充质干细胞（MSC）研究中一般使用何种生长因子？

用于MSC培养和自我更新的生长因子：LIF（PHC9484），bFGF（PHG0261），EGF（PHG0311），HGF（PHG0324），PDGF（PHG0043）和Wnt3。
用于MSC分化的生长因子或细胞因子：BMP-2（PHC7145）与TGF-β1（PHG9211）。
(Arthritis Res Ther 9:204 (2007)).

我如何在体外培养间充质干细胞（MSC）？

MSC可培养在含或不含血清的培养基条件下。标准的培养条件为含10% FBS的低葡糖DMEM。MSC也可培养于低减血清（2%）的Gibco MesenPRO RS培养基（货号12746-012），或无血清的Gibco StemPro MSC SFM XenoFree培养基（货号A10675）和Gibco StemPro MSC SFM（货号A10332）中。通常情况下，MSC在含氧量较低的条件下（2% O2）生长得较好（2% O2）。

你们如何鉴定人间充质干细胞（MSC）？

可通过流式细胞术来鉴定人体MSC，这些细胞通常具有CD73+，CD90+，CD105+，CD11b-和CD45-的标志物属性（Blood 109:4245 (2007)）。人MSC表达Oct4，Sox2和Rex-1；这些mRNA均可通过RT-PCR技术进行鉴定（Arthritis Res Ther 9:204 (2007)）。

什么是间充质干细胞（MSC）？

间充质干细胞（MSC）是最初从骨髓或脂肪组织中分离出来的多专能细胞，它们表现出进一步分化为骨，软骨和脂肪细胞的能力。在常规细胞培养条件下，从骨髓中分离出的MSC为纺锤形，并拥有贴附于未包被塑料培养皿上的独特能力（Arthritis Res Ther 9:204 (2007)）。

What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

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How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

How do you characterize human mesenchymal stem cells (MSCs)?

Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).

What are mesenchymal stem cells?

Mesenchymal stem cells (MSCs) are multipotent cells isolated primarily from bone marrow or fat tissues that exhibit the ability to differentiate into bone, cartilage, and fat cells. Under normal cell culture conditions, MSCs isolated from bone marrow are spindle shaped with the unique ability to adhere to uncoated plastic culture dishes (Arthritis Res Ther 9:204 (2007)).