FreeStyle™ CHO-S Cells - FAQs

查看更多产品信息 FreeStyle™ CHO-S Cells - FAQs (R80007)

3 个常见问题解答

从抗体和蛋白得率的角度来看，FreeStyle 293-F细胞与FreeStyle CHO-S细胞相比哪个更好？

当比较FreeStyle 293-F细胞与FreeStyle CHO-S细胞的平均抗体和蛋白得率时，绝对是FreeStyle 293-F细胞在瞬转条件下的抗体得率会比FreeStyle CHO-S细胞高。而另一方面，稳转CHO细胞的抗体得率会更高。对于非抗体蛋白而言，我们无法预测这两株细胞系的得率哪个更高。

How do FreeStyle 293-F cells compare to FreeStyle CHO-S cells in terms of antibody and protein yield?

When comparing FreeStyle 293-F cells with FreeStyle CHO-S cells for average range of antibody and protein yield, it is definitely expected that antibody yield will be higher in FreeStyle 293-F cells than in FreeStyle CHO-S cells, when generated by transient transfection. On the other hand, antibody yields in stable CHO cells can be much higher. For non-antibody proteins, it is impossible to predict which of the two cell lines may provide higher yields.

How long can I keep culturing FreeStyle cells after transfection?

We typically keep the culture going for 7-10 days posttransfection. After that, protein expression may drop.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.