ProBond™ 镍螯合树脂
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引用和文献 (5)
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ProBond™ 镍螯合树脂

ProBond™ 镍螯合树脂是一种镍荷电亲和树脂，用于纯化含多聚组氨酸 (6xHis) 序列的重组蛋白。与树脂结合的蛋白可使用低 pH 值缓冲液或通过与咪唑或组氨酸竞争进行洗脱。在天然和变性条件下均可进行一步纯化。ProBond™ 树脂使用螯合配体亚氨基二乙酸了解更多信息
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货号数量
R80115150 mL
R8010150 mL
R80115TS
又称 R80115
货号 R80115
价格（CNY)
14,980.00
Each
添加至购物车
数量:
150 mL
请求批量或定制报价
价格（CNY)
14,980.00
Each
添加至购物车
ProBond™ 镍螯合树脂是一种镍荷电亲和树脂，用于纯化含多聚组氨酸 (6xHis) 序列的重组蛋白。与树脂结合的蛋白可使用低 pH 值缓冲液或通过与咪唑或组氨酸竞争进行洗脱。在天然和变性条件下均可进行一步纯化。ProBond™ 树脂使用螯合配体亚氨基二乙酸 (IDA)，该配体偶联至适用于 FPLC、批次和重力流应用的高度交联 6% 琼脂糖树脂。
仅供科研使用。不可用于诊断程序。
规格
数量150 mL
固定相镍螯合
色谱柱类型亲和柱
形式悬浮
产品线ProBond
类型树脂
Unit SizeEach
内容与储存
预先装入 ProBond™ 树脂，每 1 mL 树脂能够结合 1-5 mg 重组蛋白。其以含 50% 浆液的 20% 乙醇溶液的形式提供。带有双价镍电荷 (Ni2+) 时，树脂将呈蓝色。在 +4°C 下储存。妥善储存时，保证 ProBond™ 树脂稳定储存 6 个月。

常见问题解答 (FAQ)

你们通常怎样检测重组融合蛋白的表达？

通常使用免疫印迹分析法检测蛋白表达。我们提供许多针对不同表位的抗体，如Xpress、HisG、V5或C-端6xHis。此外，可以用我们的ProBond亲和纯化系统来纯化His标签蛋白。

How do you typically detect expression of a recombinant fusion protein?

Western blot analysis is typically used to detect the expressed protein. We sell several antibodies against various epitopes, such as Xpress, HisG, V5, or C-terminal 6xHis. Additionally, His-tagged proteins can be purified using our ProBond Purification System via affinity purification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What expression levels can be expected with the pTrcHis/CAT construct?

In one experiment, 35 µg CAT/mg total protein or 68.2 mg CAT/liter of culture was obtained. 50 ml of cell extract was loaded onto a ProBond column and 2 mg of CAT was recovered. The eluted protein appeared reasonably pure on a gel.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

I purified my protein from a ProBond column using denaturing conditions. After elution, I tried digesting off my N- terminal tag with EKMax Enterokinase, but see no EK cleavage. What can you suggest I try?

The enzyme could be denatured. Try buffer exchange or dialysis before digestion with EKMax Enterokinase.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all ProBond™ 镍螯合树脂 FAQs

引用和文献 (5)

引用和文献
Abstract
Total synthesis of cyclic ADP-carbocyclic-ribose, a stable mimic of Ca2+-mobilizing second messenger cyclic ADP-ribose.
Authors: Shuto S; Fukuoka M; Manikowsky A; Ueno Y; Nakano T; Kuroda R; Kuroda H; Matsuda A;
Journal:J Am Chem Soc
PubMed ID:11535079
'The synthesis of cyclic ADP-carbocyclic-ribose (cADPcR, 4) designed as a stable mimic of cyclic ADP-ribose (cADPR, 1), a Ca2+-mobilizing second messenger, was achieved using as the key step a condensation reaction with the phenylthiophosphate-type substrate 14 to form an intramolecular pyrophosphate linkage. The N-1-carbocyclic-ribosyladenosine derivative 16 was prepared via the ... More
Interaction between the insulin receptor and its downstream effectors. Use of individually expressed receptor domains for structure/function analysis.
Authors:Paz K, Voliovitch H, Hadari YR, Roberts CT Jr, LeRoith D, Zick Y
Journal:J Biol Chem
PubMed ID:8636129
'A structural analysis has been carried out to determine which part of the intracellular domain of the insulin receptor (IR) beta subunit is involved in direct interaction with the receptor substrates IRS-1 and Shc. Toward this end, the juxtamembrane (JM) domain (amino acids 943- 984) and the carboxyl-terminal (CT) region ... More
A Calcium-Responsive Transcription Factor, CaRF, that Regulates Neuronal Activity-Dependent Expression of BDNF.
Authors: Tao Xu; West Anne E; Chen Wen G; Corfas Gabriel; Greenberg Michael E;
Journal:Neuron
PubMed ID:11832226
'Transcription of the brain-derived neurotrophic factor (BDNF) gene is regulated in a calcium- and neuron-selective manner; however, the mechanisms that underlie this selectivity are not known. We have characterized a new calcium-response element, CaRE1, that is required for activity-dependent transcription of BDNF exon III and have cloned a transcription factor, ... More
Tryptophan fluorescence reports nucleotide-induced conformational changes in a domain of the ArsA ATPase.
Authors:Zhou T, Rosen BP
Journal:J Biol Chem
PubMed ID:9242630
The ars operon of plasmid R773 encodes an ATP-dependent extrusion pump for arsenite and antimonite in Escherichia coli. The ArsA ATPase is the catalytic subunit of the pump protein, with two nucleotide binding consensus sequences, one in the NH2-terminal half and one in the COOH- terminal half of the protein. ... More
Structure and ligand of a histone acetyltransferase bromodomain.
Authors:Dhalluin C, Carlson JE, Zeng L, He C, Aggarwal AK, Zhou MM
Journal:Nature
PubMed ID:10365964
Histone acetylation is important in chromatin remodelling and gene activation. Nearly all known histone-acetyltransferase (HAT)-associated transcriptional co-activators contain bromodomains, which are approximately 110-amino-acid modules found in many chromatin-associated proteins. Despite the wide occurrence of these bromodomains, their three-dimensional structure and binding partners remain unknown. Here we report the solution structure ... More