Oxichrome Reagent

Rapidly detect the enzyme cytochrome oxidase in microorganisms using Thermo Scientific™ Remel™ Oxichrome Reagent. The oxidase test was originally developed to identify Neisseria spp., but later was used to separate pseudomonads from oxidase-negative enteric Gram-negative bacilli (Enterobacteriaceae)^1,2. Most Gram-positive bacteria are oxidase-negative, pseudomonads are oxidase-positive, and other non-enteric, Gram-negative bacilli, are variable

Pseudomonads, bacteria of the family Pseudomonadaceae are a large and varied group comprising four major genera and several hundred species. Most species of the family are found in soil or water; some cause diseases in plants, and a few cause serious diseases in humans and other mammals. Pseudomonas aeruginosa, very common and widespread, is an opportunistic pathogen for man that causes antibiotic-resistive infections in persons of weakened resistance.

The oxidase reaction is attributed to the presence of a cytochrome oxidase system in the respiratory chain of certain bacteria, which utilizes molecular oxygen as a terminal electron acceptor^3,4. Oxidase-positive bacteria produce oxidase enzymes which, in the presence of atmospheric oxygen, cytochrome c, and an oxidase reagent (Oxichrome Reagent), oxidize the reagent to form a blue-purple colored compound, Wurster’s blue.

• Ready-to-use – Liquid reagent
• Easy to interpret – Blue to purple color indicates a positive test
• Easy-to-store – Storage at room temperature
• Rapid results – Can be interpreted within 30 seconds

Oxichrome reagent contains N,N,N,N-tetramethyl-ρ-phenylenediamine supplied in 15 mL bottles.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.