HisG Epitope Tag Monoclonal Antibody - FAQs

查看更多产品信息 HisG Epitope Tag Monoclonal Antibody - FAQs (R94025)

3 个常见问题解答

Can the Anti-His (C-term) antibody be used against the N-terminal HisG (His-His-His-His-His-His-Gly) epitope tag?

You cannot use Anti-His (C-term) antibody against the N-terminal HisG epitope taq. Thermo Fisher Scientific sells two Anti-His antibodies: Anti-HisG and Anti-His (C-term) antibodies depending on the epitope tag in your vector. These antibodies do not recognize react well with both epitopes because the glycine and the carboxy terminus are important parts of each one. Any 6xHis tag lacking in these additional elements is a poor epitope for either of the antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Will the Anti-HisG antibody recognize proteins made in pDEST17 (N-terminal His tag vector)?

No, the Anti-HisG antibody detects the N-terminal polyhistidine (6x His) tag followed by glycine: HHHHHHG. The proteins made from the pDEST17 vectors do not have this glycine following the His tag and therefore will not be recognized by this antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What applications are appropriate for your Anti-Xpress antibody, Anti-V5 antibody, Anti-HisG antibody, and Anti-myc antibody?

ELISA, Western blot, Immunoprecipitation, Immunofluorescence, and Immunohistochemistry protocols have all been used successfully with our epitope tag antibodies. Please consult the product manuals for specific protocols and dilutions.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.