HisG Epitope Tag Monoclonal Antibody, AP - FAQs

查看更多产品信息 HisG Epitope Tag Monoclonal Antibody, AP - FAQs (R94225)

4 个常见问题解答

你们通常怎样检测重组融合蛋白的表达？

通常使用免疫印迹分析法检测蛋白表达。我们提供许多针对不同表位的抗体，如Xpress、HisG、V5或C-端6xHis。此外，可以用我们的ProBond亲和纯化系统来纯化His标签蛋白。

我能否使用anti-HisG抗体和免疫印迹来检测Gateway pT-REx DEST31载体中的His标签？

Gateway pT-REx DEST31载体包含了N-端的6xHis标签（这一载体中6XHis标签后面未跟随甘氨酸（G）或-COOH）。因此，这一标签无法通过anti-HisG或anti-His（C端）抗体实现检测。作为替代，我们推荐使用anti-6xHis抗体（货号 372900）。

How do you typically detect expression of a recombinant fusion protein?

Western blot analysis is typically used to detect the expressed protein. We sell several antibodies against various epitopes, such as Xpress, HisG, V5, or C-terminal 6xHis. Additionally, His-tagged proteins can be purified using our ProBond Purification System via affinity purification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use the anti-HisG antibody for western detection of the His tag in Gateway pT-REx DEST31?

The Gateway pT-REx DEST31 vector contains an N-terminal 6xHis tag (the 6XHis in this vector is not followed by Glycine (G) or -COOH). Hence, it cannot be detected using anti-HisG or anti-His (C-terminal) antibodies. Instead, we recommend using an anti-6xHis antibody (Cat. No. 372900).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.