Characterization of c-kit positive intrathymic stem cells that are restricted to lymphoid differentiation.
AuthorsMatsuzaki Y, Gyotoku J, Ogawa M, Nishikawa S, Katsura Y, Gachelin G, Nakauchi H
JournalJ Exp Med
PubMed ID7690832
We found that c-kit-positive, lineage marker-negative, Thy-1lo cells are present in both bone marrow and thymus ("BM c-kit" and "thymus c-kit" cells). Although the two cell types are phenotypically similar, only BM c-kit cells showed the potential to form colonies in vitro as well as in vivo. However, both of ... More
c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody.
AuthorsRico-Vargas SA, Weiskopf B, Nishikawa S, Osmond DG
JournalJ Immunol
PubMed ID7511630
To examine the in vivo role of c-kit receptor in B lymphopoiesis we have evaluated precursor B cell populations expressing c-kit in mouse bone marrow and the effects on B cell genesis of administering a neutralizing anti-c-kit mAb, ACK2. Double immunofluorescence labeling and mitotic arrest were used to examine bone ... More
Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation.
AuthorsIkuta K, Weissman IL
JournalProc Natl Acad Sci U S A
PubMed ID1371359
The interaction of the mouse c-kit receptor, designated Kit receptor, and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. Monoclonal antibodies against the extracellular portion of the mouse Kit receptor were established. Five percent to 10% of total bone marrow cells expressed the Kit receptor, and half ... More