RPCI22.P - FAQs

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2 个常见问题解答

对我感兴趣的基因，使用基因组BAC克隆代替cDNA克隆的原因是什么？

BAC克隆为包含很大插入片段（>100 kb）的基因组克隆，可能包含几个基因，而cDNA克隆只包含一个开放读码框基因。BAC克隆适用于启动子及内含子／外显子分析，染色体定位及转基因小鼠制备。

What are some reasons why I should pick a genomic BAC clone for my gene instead of a cDNA clone?

BAC clones are genomic clones containing very large inserts (>100 kb) that may contain several genes, whereas cDNA clones only contain a single open reading frame of a gene. BAC clones are used for promoter and intron/exon analysis, chromosome localization, and making transgenic mice.