Enzyme-mediated methodology for the site-specific radiolabeling of antibodies based on catalyst-free click chemistry.
AuthorsZeglis BM, Davis CB, Aggeler R, Kang HC, Chen A, Agnew BJ, Lewis JS,
Journal
PubMed ID23688208
'An enzyme- and click chemistry-mediated methodology for the site-selective radiolabeling of antibodies on the heavy chain glycans has been developed and validated. To this end, a model system based on the prostate specific membrane antigen-targeting antibody J591, the positron-emitting radiometal (89)Zr, and the chelator desferrioxamine has been employed. The methodology ... More