SiteClick™ Antibody Azido Modification Kit, for 5 mg of Ab

SiteClick™ Antibody Azido Modification Kit, for 5 mg of Ab - FAQs

查看更多产品信息 SiteClick™ Antibody Azido Modification Kit - FAQs (S10901)

4 个常见问题解答

Does the BSA content have any influence on labeling antibodies with the SiteClick Antibody Azido Modification Kit (Cat. No. S10901)?

No, the presence of BSA will not influence the labeling efficiency of the SiteClick Kit. Glycoproteins at a high concentration may affect the labeling efficiency and since BSA is not a glycoprotein, it does not inhibit labeling efficiency with the UDP-GalNAz component of the SiteClick kit.

Find additional tips, troubleshooting help, and resources within our Labeling Chemistry Support Center.

Once I label my antibody with azide using SiteClick Antibody Azido Modification Kit, can it be labeled using any alkyne, DIBO, or sDIBO alkyne?

Yes. We highly recommend using an sDIBO alkyne in order to limit non-specific binding to the antibody, but the azide would react with any alkyne (using copper-mediated click reaction) or any DIBO or sDIBO alkyne (using a copper-free click reaction).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you perform SiteClick labeling of antibodies as a custom service?

Yes. See this web link: https://www.thermofisher.com/us/en/home/life-science/antibodies/antibody-labeling/custom-fluorescent-antibody-protein-labeling.html

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use SiteClick Antibody Azido Modification Kit to label other proteins, not antibodies?

We do not recommend doing this. Although the sDIBO alkynes will react with any azide-modified molecule, SiteClick Antibody Azido Modification Kit was optimized specifically for antibody labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.