SiteClick™ Biotin sDIBO Alkyne, for 1 mg of Ab - FAQs

查看更多产品信息 SiteClick™ sDIBO Alkyne Kits for Antibody Labeling - FAQs (S10904, S10915, S10903, S10914, S10902, S10908, S10907, C20031, S10906, C20030, S10911, C20034, S10909, C20029, C20028, C20027)

3 个常见问题解答

How is SiteClick Biotin sDIBO Alkyne different from Click-iT Biotin sDIBO Alkyne?

Structurally, they are the same. The only difference is in the manner of packaging. SiteClick Biotin sDIBO Alkyne is packaged for labeling 1 to 5 mg of antibody per the SiteClick labeling protocol with the actual amount being proprietary. Click-iT Biotin sDIBO Alkyne is packaged as a 0.5 mg of reagent for use with any copper-free click reaction.

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What is the general protocol for using the sDIBO alkynes?

The protocol is very simple. Expose the azide-modified sample to the sDIBO alkyne (final concentration in the range of 1 to 5 µM is a reasonable starting range) in aqueous buffer of media for 1-2 hrs. Optimize the final working concentration and incubation time per the sample/application. The azide-modified target must have the azide accessible for reacting with the sDIBO, and the labeling solution should not include any sodium azide.

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How are the sDIBO alkynes different from the original DIBO alkynes?

Both the original DIBO alkynes and the sDIBO alkynes utilize the same fluorescent dyes or biotin for detection. The sDIBO alkynes have improved solubility in aqueous solutions and less propensity to bind non-specifically compared to the original DIBO alkynes.

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