SiteClick™ pHrodo™ iFL Red sDIBO Alkyne, for 1 mg of Ab - FAQs

查看更多产品信息 SiteClick™ sDIBO Alkyne Kits for Antibody Labeling - FAQs (S10904, S10915, S10903, S10914, S10902, S10908, S10907, C20031, S10906, C20030, S10911, C20034, S10909, C20029, C20028, C20027)

3 个常见问题解答

You only offer a pHrodo Red sDIBO alkyne (Cat. Nos. S10903, S10908). Is it possible to get pHrodo Green or other dyes as an sDIBO derivative?

Yes. This is possible through a custom order.

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What is the general protocol for using the sDIBO alkynes?

The protocol is very simple. Expose the azide-modified sample to the sDIBO alkyne (final concentration in the range of 1 to 5 µM is a reasonable starting range) in aqueous buffer of media for 1-2 hrs. Optimize the final working concentration and incubation time per the sample/application. The azide-modified target must have the azide accessible for reacting with the sDIBO, and the labeling solution should not include any sodium azide.

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How are the sDIBO alkynes different from the original DIBO alkynes?

Both the original DIBO alkynes and the sDIBO alkynes utilize the same fluorescent dyes or biotin for detection. The sDIBO alkynes have improved solubility in aqueous solutions and less propensity to bind non-specifically compared to the original DIBO alkynes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.