SiteClick™ pHrodo™ Deep Red sDIBO Alkyne, For up to 250 μg of Ab - FAQs

查看更多产品信息 SiteClick™ sDIBO Alkyne Kits for Antibody Labeling - FAQs (S10904, S10915, S10903, S10914, S10902, S10908, S10907, C20031, S10906, C20030, S10911, C20034, S10909, C20029, C20028, C20027)

3 个常见问题解答

May I store antibodies or other proteins labeled with SiteClick sDIBO Alkyne Kit, pHrodo Deep Red (Cat. Nos. S10914, S10915) in solutions containing sodium azide?

We do not recommend storing antibodies or proteins labeled with SiteClick sDIBO Alkyne Kit, pHrodo Deep Red (Cat. Nos. S10914, S10915) in solutions containing sodium azide, as pHrodo Deep Red is not stable in the presence of sodium azide. Consider using an alternative, non-azide-based anti-microbial.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How can I perform a DOL determination for SiteClick sDIBO Alkyne Kit, pHrodo Deep Red (Cat. Nos. S10914, S10915)?

To determine the Degree of Labeling (DOL) for the SiteClick sDIBO Alkyne Kit, pHrodo Deep Red (Cat. Nos. S10914, S10915), refer to page 13 of the SiteClick Antibody Azido Modification Kit User Guide.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

May I use the SiteClick sDIBO Alkyne Kit, pHrodo Deep Red (Cat. Nos. S10914, S10915), to label any azide-modified protein besides antibodies?

Yes. You can use the SiteClick sDIBO Alkyne Kit, pHrodo Deep Red (Cat. Nos. S10914, S10915), to label any azide-modified protein as long as the azide moiety is accessible. You may perform a copper-free click reaction to label the protein with the sDIBO alkynes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.