链霉亲和素，Pacific Blue™ 偶联物

引用和文献 (8)

链霉亲和素，Pacific Blue™ 偶联物

我们的链霉素亲和素 Pacific Blue™ 偶联物是一种明亮的蓝色荧光探针，激发/发射波长分别为 ∼410/455 nm。链霉亲和素是一种非糖基化生物素结合蛋白，从细菌中分离所得，具有近中性等电点。由于经报道其非特异性结合比例比糖基化生物素结合蛋白（亲和素）更低，故广泛用于检测生物素化探针了解更多信息

货号	数量
S11222	1 mg

货号 S11222

价格（CNY)

4,603.00

添加至购物车

价格（CNY)

4,603.00

添加至购物车

我们的链霉素亲和素 Pacific Blue™ 偶联物是一种明亮的蓝色荧光探针，激发/发射波长分别为 ∼410/455 nm。链霉亲和素是一种非糖基化生物素结合蛋白，从细菌中分离所得，具有近中性等电点。由于经报道其非特异性结合比例比糖基化生物素结合蛋白（亲和素）更低，故广泛用于检测生物素化探针。

查看所有 Alexa Fluor™ 链霉亲和素-亲和素偶联物选择指南。

仅供科研使用。不可用于诊断程序。

描述Pacific Blue™ 偶联物链霉素亲和素，1mg，黄色，固体形式，407nm 吸光度，Pacific Blue™ 标签或染料，410/455nm 激发/发射波长，用于印迹分析、流式细胞分析、组化应用、免疫检测，储存温度为 –5 到 –30°C

检测方法荧光

激发/发射410/455 nm

流式细胞仪激光线路405

适用于（应用）流式细胞分析、免疫检测、组化应用、印迹分析

标签或染料Pacific Blue

包装1/包

产品类型链霉素亲和素偶联物

纯度或质量等级薄层层析

数量1 mg

调控状态仅供研究使用 (RUO)

样品体积1 mg

有效期每 6 个月

运输条件室温

储存缓冲液10mg/mL PBS

偶联物Pacific Blue

产品线Pacific Blue

Unit SizeEach

内容与储存

包含 1 小瓶链霉素亲和素 Pacific Blue™ 偶联物。

在冰箱（-5 至 -30°C）中避光储存。

常见问题解答 (FAQ)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

引用和文献 (8)

引用和文献

Abstract

Arenavirus nucleoprotein targets interferon regulatory factor-activating kinase IKKe.

Authors:Pythoud C, Rodrigo WW, Pasqual G, Rothenberger S, Martínez-Sobrido L, de la Torre JC, Kunz S,

Journal:J Virol

PubMed ID:22532683

'Arenaviruses perturb innate antiviral defense by blocking induction of type I interferon (IFN) production. Accordingly, the arenavirus nucleoprotein (NP) was shown to block activation and nuclear translocation of interferon regulatory factor 3 (IRF3) in response to virus infection. Here, we sought to identify cellular factors involved in innate antiviral signaling ... More

In vivo expansion of T reg cells with IL-2-mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression.

Authors:Webster KE, Walters S, Kohler RE, Mrkvan T, Boyman O, Surh CD, Grey ST, Sprent J,

Journal:J Exp Med

PubMed ID:19332874

'Via a transcription factor, Foxp3, immunoregulatory CD4(+)CD25(+) T cells (T reg cells) play an important role in suppressing the function of other T cells. Adoptively transferring high numbers of T reg cells can reduce the intensity of the immune response, thereby providing an attractive prospect for inducing tolerance. Extending our ... More

Rapid lytic granule convergence to the MTOC in natural killer cells is dependent on dynein but not cytolytic commitment.

Authors:Mentlik AN, Sanborn KB, Holzbaur EL, Orange JS,

Journal:Mol Biol Cell

PubMed ID:20444980

'Natural killer cells are lymphocytes specialized to participate in host defense through their innate ability to mediate cytotoxicity by secreting the contents of preformed secretory lysosomes (lytic granules) directly onto a target cell. This form of directed secretion requires the formation of an immunological synapse and occurs stepwise with actin ... More

Immunosuppressive property within the Streptococcus pneumoniae cell wall that inhibits generation of T follicular helper, germinal center, and plasma cell response to a coimmunized heterologous protein.

Authors:Saumyaa, Arjunaraja S, Pujanauski L, Colino J, Torres RM, Snapper CM,

Journal:

PubMed ID:23817619

'We previously demonstrated that intact, inactivated Streptococcus pneumoniae (unencapsulated strain R36A) inhibits IgG responses to a number of coimmunized soluble antigens (Ags). In this study, we investigated the mechanism of this inhibition and whether other extracellular bacteria exhibited similar effects. No inhibition was observed if R36A was given 24 h ... More

Thymocytes may persist and differentiate without any input from bone marrow progenitors.

Authors:Peaudecerf L, Lemos S, Galgano A, Krenn G, Vasseur F, Di Santo JP, Ezine S, Rocha B,

Journal:J Exp Med

PubMed ID:22778388

'Thymus transplants can correct deficiencies of the thymus epithelium caused by the complete DiGeorge syndrome or FOXN1 mutations. However, thymus transplants were never used to correct T cell-intrinsic deficiencies because it is generally believed that thymocytes have short intrinsic lifespans. This notion is based on thymus transplantation experiments where it ... More

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