Regulation of chemotactic and proadhesive responses to chemoattractant receptors by RGS (regulator of G-protein signaling) family members.
AuthorsBowman EP, Campbell JJ, Druey KM, Scheschonka A, Kehrl JH, Butcher EC
JournalJ Biol Chem
PubMed ID9774420
'Serpentine Galphai-linked receptors support rapid adhesion and directed migration of leukocytes and other cell types. The intracellular mechanisms mediating and regulating chemoattractant-directed adhesion and locomotion are only now beginning to be explored. RGS (for regulator of G-protein signaling) proteins are a recently described family that regulate Galphai-stimulated pathways by acting ... More
Vertical movement of epithelial basal cells toward the corneal surface during use of extended-wear contact lenses.
AuthorsLadage PM, Jester JV, Petroll WM, Bergmanson JP, Cavanagh HD
JournalInvest Ophthalmol Vis Sci
PubMed ID12601029
'PURPOSE: To study the effects of extended contact lens wear (EW) on the movement of basal epithelial cells toward the corneal surface. METHODS: Rabbits (n = 32) were injected with 5-bromo-2-deoxyuridine (BrdU) to label a group of proliferating basal epithelial cells, and, 24 hours later, one randomly chosen eye was ... More
Multiparameter detection of apoptosis using red-excitable SYTO probes.
'Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent ... More
Liver fatty acid-binding protein colocalizes with peroxisome proliferator activated receptor alpha and enhances ligand distribution to nuclei of living cells.
AuthorsHuang H, Starodub O, McIntosh A, Atshaves BP, Woldegiorgis G, Kier AB, Schroeder F
JournalBiochemistry
PubMed ID14992586
'Although it is hypothesized that long-chain fatty acyl CoAs (LCFA-CoAs) and long-chain fatty acids (LCFAs) regulate transcription in the nucleus, little is known regarding factors that determine the distribution of these ligands to nuclei of living cells. Immunofluorescence colocalization showed that liver fatty acid-binding protein (L-FABP; binds LCFA-CoA as well ... More
Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry.
AuthorsDay JP, Kell DB, Griffith GW
JournalAppl Environ Microbiol
PubMed ID11772606
'The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or ... More
The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria.
AuthorsGuy R, Liu P, Pennefather P, Crandall I,
JournalMalar J
PubMed ID17617912
'BACKGROUND: Giemsa staining of thick blood smears remains the "gold standard" for detecting malaria. However, this method is not very good for diagnosing low-level infections. A method for the simultaneous staining of Plasmodium-parasitized culture and blood smears for both bright field and fluorescence was developed and its ability to improve ... More
A calpain unique to alveolates is essential in Plasmodium falciparum and its knockdown reveals an involvement in pre-S-phase development.
AuthorsRusso I, Oksman A, Vaupel B, Goldberg DE,
JournalProc Natl Acad Sci U S A
PubMed ID19164769
'Plasmodium falciparum encodes a single calpain that has a distinct domain composition restricted to alveolates. To evaluate the potential of this protein as a drug target, we assessed its essentiality. Both gene disruption by double cross-over and gene truncation by single cross-over recombination failed. We were also unable to achieve ... More
pEYFP-Nuc vector is a useful tool for three-dimensional and time-lapse observation of nuclear morphology of Jurkat cells during apoptosis.
AuthorsHasegawa A, Kuroda M, Wang Y, Akaki S, Asaumi J, Shibuya K, Kawasaki S, Okumura Y, Kanazawa S, Hiraki Y
JournalInt J Mol Med
PubMed ID14719129
'Using a pEYFP-Nuc vector, which contains nucleic acid sequences of a nuclear localization signal, we established a Jurkat-YN cell line that expressed enhanced yellow fluorescent protein (EYFP) in the nucleus. We observed three-dimensional and time-lapse changes in nuclear morphology of Jurkat-YN cells during Fas-induced apoptosis using a confocal laser scanning ... More
Comparison of animal infectivity and nucleic acid staining for assessment of Cryptosporidium parvum viability in water.
AuthorsNeumann NF, Gyürek LL, Gammie L, Finch GR, Belosevic M
JournalAppl Environ Microbiol
PubMed ID10618255
Cryptosporidium parvum oocysts were stained with the fluorogenic dyes SYTO-9 and SYTO-59 and sorted by flow cytometry in order to determine whether the fluorescent staining intensity correlated with the ability of oocysts to infect neonatal CD-1 mice. Oocysts that did not fluoresce or that displayed weak fluorescent intensity when stained ... More
Glucose Directly Links to Lipid Metabolism through High Affinity Interaction with Peroxisome Proliferator-activated Receptor {alpha}.
AuthorsHostetler HA, Huang H, Kier AB, Schroeder F,
JournalJ Biol Chem
PubMed ID18055466
The pathophysiology of diabetes is characterized not only by elevated glucose but also elevated long chain fatty acid levels. We show for the first time that the peroxisome proliferator-activated receptor-alpha (PPARalpha) binds glucose and glucose metabolites with high affinity, resulting in significantly altered PPARalpha secondary structure. Glucose decreased PPARalpha interaction ... More
Nucleic acid stains as indicators of Cryptosporidium parvum oocyst viability.
AuthorsBelosevic M, Guy RA, Taghi-Kilani R, Neumann NF, Gyürék LL, Liyanage LR, Millard PJ, Finch GR
JournalInt J Parasitol
PubMed ID9279581
We developed nucleic acid dye staining methodology for untreated, heat-treated and chemically inactivated C. parvum oocysts. The nucleic acid staining was compared to in vitro excystation and animal infectivity using split samples of oocysts. Among the nucleic acid stains tested, SYTO-9, hexidium and SYTO-59 stained the oocysts consistently, and the ... More
Organization and dynamics of growing microtubule plus ends during early mitosis.
AuthorsPiehl M, Cassimeris L
JournalMol Biol Cell
PubMed ID12631713
A stable cell line expressing EB1-green fluorescent protein was used to image growing microtubule plus ends at the G(2)/M transition. By late prophase growing ends no longer extend to the cell periphery and were not uniformly distributed around each centrosome. Growing ends were much more abundant in the area surrounding ... More
Confocal imaging of microglial cell dynamics in hippocampal slice cultures.
AuthorsDailey ME, Waite M
JournalMethods
PubMed ID10356354
Methods are described for imaging the cellular dynamics of microglia in live mammalian brain slice cultures. Brain slices prepared from developing rat hippocampus are cultured for up to 2 weeks by the roller tube or static filter culture technique, stained with one or more fluorescent dyes, and imaged by scanning ... More
The phosphoinositol-3-kinase-protein kinase B/Akt pathway is critical for Pseudomonas aeruginosa strain PAK internalization.
AuthorsKierbel A, Gassama-Diagne A, Mostov K, Engel JN
JournalMol Biol Cell
PubMed ID15772151
Several Pseudomonas aeruginosa strains are internalized by epithelial cells in vitro and in vivo, but the host pathways usurped by the bacteria to enter nonphagocytic cells are not clearly understood. Here, we report that internalization of strain PAK into epithelial cells triggers and requires activation of phosphatidylinositol 3-kinase (PI3K) and ... More
TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual-colour FISH on paraffin sections.
AuthorsBink K, Walch A, Feuchtinger A, Eisenmann H, Hutzler P, Höfler H, Werner M
JournalHistochem Cell Biol
PubMed ID11405057
Confocal laser scanning microscopy (CLSM) is an extensive but reliable tool for assessing the hybridisation signals in fluorescence in situ hybridisation (FISH). Most CLSMs are equipped with an argon-laser and a helium/neon-laser illumination system with excitation wavelengths of 488, 543 and 633 nm. A protocol for an optimal nuclear counterstaining ... More
Nuclear trafficking of photoreceptor protein crx: the targeting sequence and pathologic implications.
AuthorsFei Y, Hughes TE
JournalInvest Ophthalmol Vis Sci
PubMed ID10967037
PURPOSE. To identify the targeting sequence controlling the nuclear transport of the photoreceptor-specific transcription factor cone-rod homeobox (Crx) protein and to address the question of whether disease-causing Crx mutations disrupt the nuclear trafficking of the Crx protein. METHODS. A series of cDNA fragments encoding Crx protein with deleted C termini ... More
Fluorescent labeling of blood cells for evaluation of retinal and choroidal circulation.
AuthorsKhoobehi B, Peyman GA
JournalOphthalmic Surg Lasers
PubMed ID10037209
BACKGROUND AND OBJECTIVE: To develop a method of staining and tracking cells in vivo with a scanning laser ophthalmoscope (SLO) to evaluate the retinal and choroidal circulations. MATERIALS AND METHODS: Twelve pigmented male rats were used. Staining of leukocytes. Two commercially available nucleic acid stains SYTO 16 and SYTO 59 ... More
Host and bacterial proteases influence biofilm formation and virulence in a murine model of enterococcal catheter-associated urinary tract infection.
Authors
JournalNPJ Biofilms Microbiomes
PubMed ID29134108
Neural Processing of Communication Signals: The Extent of Sender-Receiver Matching Varies across Species of Apteronotus.
Authors
JournalEneuro
PubMed ID30899777
The N-Acetylglucosaminidase LytB of Streptococcus pneumoniae Is Involved in the Structure and Formation of Biofilms.
Authors
JournalAppl Environ Microbiol
PubMed ID32198170
Mucus threads from surface goblet cells clear particles from the airways.
Authors
JournalRespir Res
PubMed ID34823518
Development of a glial network in the olfactory nerve: role of calcium and neuronal activity.