The protein tyrosine phosphatase PTP-BL associates with the midbody and is involved in the regulation of cytokinesis.
AuthorsHerrmann L, Dittmar T, Erdmann KS
JournalMol Biol Cell
PubMed ID12529439
'PTP-BL is a highly modular protein tyrosine phosphatase of unknown function. It consists of an N-terminal FERM domain, five PDZ domains, and a C-terminally located tyrosine phosphatase domain. Here we show that PTP-BL is involved in the regulation of cytokinesis. We demonstrate localization of endogenous PTP-BL at the centrosomes during ... More
Nucleic acid binding agents exert local toxic effects on neurites via a non-nuclear mechanism.
AuthorsPin S, Chen H, Lein PJ, Wang MM
JournalJ Neurochem
PubMed ID16441515
'The mechanism by which drugs that target nucleic acids cause neurotoxicity is not well described. We characterized the neurotoxicity of Hoechst 33342 (bis-benzimide), a common cell permeable nuclear dye, in primary neuronal cultures. The mechanism of cell death was not apoptotic, as death is rapid, not accompanied by typical nuclear ... More
Multiparameter detection of apoptosis using red-excitable SYTO probes.
'Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent ... More
Liver fatty acid-binding protein colocalizes with peroxisome proliferator activated receptor alpha and enhances ligand distribution to nuclei of living cells.
AuthorsHuang H, Starodub O, McIntosh A, Atshaves BP, Woldegiorgis G, Kier AB, Schroeder F
JournalBiochemistry
PubMed ID14992586
'Although it is hypothesized that long-chain fatty acyl CoAs (LCFA-CoAs) and long-chain fatty acids (LCFAs) regulate transcription in the nucleus, little is known regarding factors that determine the distribution of these ligands to nuclei of living cells. Immunofluorescence colocalization showed that liver fatty acid-binding protein (L-FABP; binds LCFA-CoA as well ... More
Cytoplasmic and nuclear delivery of a TAT-derived peptide and a beta-peptide after endocytic uptake into HeLa cells.
AuthorsPotocky TB, Menon AK, Gellman SH
JournalJ Biol Chem
PubMed ID14517218
'Several short, highly cationic peptides are able to enter the cytoplasm and nucleus of cells from the extracellular medium. The mechanism of entry is unknown. A number of fluorescence-based studies suggested that these molecules cross the plasma membrane by an energy-independent process, directly gaining access to the cytoplasm. Recent reports ... More
Compatibility of the green fluorescent protein and a general nucleic acid stain for quantitative description of a Pseudomonas putida biofilm.
AuthorsNancharaiah YV, Venugopalan VP, Wuertz S, Wilderer PA, Hausner M
JournalJ Microbiol Methods
PubMed ID15590092
'Better understanding of biofilm development is essential for making optimal use of beneficial biofilms as well as for devising effective control strategies for detrimental biofilms. Analysis of biofilm structure and quantification of biofilm parameters using optical (including confocal) microscopy and digital image analysis techniques are becoming routine in many laboratories. ... More
Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry.
AuthorsDay JP, Kell DB, Griffith GW
JournalAppl Environ Microbiol
PubMed ID11772606
'The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or ... More
Fibroblast growth factor receptor 1 is a key regulator of early adipogenic events in human preadipocytes.
Cell number is an important determinant of adipose tissue mass, and the coordinated proliferation and differentiation of preadipocytes into mature lipid-laden adipocytes underpins the increased adipose tissue mass associated with obesity. Despite this, the molecular cues governing such adipose tissue expansion are poorly understood. We previously reported that fibroblast growth ... More
Multiparameter assessments to determine the effects of sugars and antimicrobials on a polymicrobial oral biofilm.
AuthorsYang Y, Sreenivasan PK, Subramanyam R, Cummins D,
JournalAppl Environ Microbiol
PubMed ID17021225
Clinical studies indicate relationships between dental plaque, a naturally formed biofilm, and oral diseases. The crucial role of nonmicrobial biofilm constituents in maintaining biofilm structure and biofilm-specific attributes, such as resistance to shear and viscoelasticity, is increasingly recognized. Concurrent analyses of the diverse nonmicrobial biofilm components for multiparameter assessments formed ... More
Functional mapping of disease susceptibility loci using cell biology.
AuthorsAntinozzi PA, Garcia-Diaz A, Hu C, Rothman JE
JournalProc Natl Acad Sci U S A
PubMed ID16537450
In most genome-wide linkage studies, implication of a causative disease gene often requires years of expanding the study to more families and finer mapping of the initially described region. Even after such efforts, unobtainable sample sizes can be required to make statistically meaningful conclusions about a single gene. Here we ... More
Macropinocytosis of protein is an amino acid supply route in Ras-transformed cells.
Authors
JournalNature
PubMed ID23665962
Recurrent SMARCB1 Mutations Reveal a Nucleosome Acidic Patch Interaction Site That Potentiates mSWI/SNF Complex Chromatin Remodeling.
Authors
JournalCell
PubMed ID31759698
Experiments from unfinished Registered Reports in the Reproducibility Project: Cancer Biology.
Authors
JournalElife
PubMed ID34874009
Combination therapy for tuberculosis treatment: pulmonary administration of ethionamide and booster co-loaded nanoparticles.
AuthorsCosta-Gouveia J, Pancani E, Jouny S, Machelart A, Delorme V, Salzano G, Iantomasi R, Piveteau C, Queval CJ, Song OR, Flipo M, Deprez B, Saint-André JP, Hureaux J, Majlessi L, Willand N, Baulard A, Brodin P, Gref R
JournalSci Rep
PubMed ID28710351
'Tuberculosis (TB) is a leading infectious cause of death worldwide. The use of ethionamide (ETH), a main second line anti-TB drug, is hampered by its severe side effects. Recently discovered "booster" molecules strongly increase the ETH efficacy, opening new perspectives to improve the current clinical outcome of drug-resistant TB. To ... More
Transite: A Computational Motif-Based Analysis Platform That Identifies RNA-Binding Proteins Modulating Changes in Gene Expression.
Authors
JournalCell Rep
PubMed ID32846122
VISAGE Reveals a Targetable Mitotic Spindle Vulnerability in Cancer Cells.
Authors
JournalCell Syst
PubMed ID31302152
The Origins and Vulnerabilities of Two Transmissible Cancers in Tasmanian Devils.
Authors
JournalCancer Cell
PubMed ID29634948
The G protein-coupled receptor GPR31 promotes membrane association of KRAS.
Authors
JournalJ Cell Biol
PubMed ID28619714
Glutamine depletion regulates Slug to promote EMT and metastasis in pancreatic cancer.
Authors
JournalJ Exp Med
PubMed ID32510550
Analysis of RAS protein interactions in living cells reveals a mechanism for pan-RAS depletion by membrane-targeted RAS binders.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID32424096
Extracellular DNA Promotes Efficient Extracellular Electron Transfer by Pyocyanin in Pseudomonas aeruginosa Biofilms.
Authors
JournalCell
PubMed ID32763156
Reproducible pharmacogenomic profiling of cancer cell line panels.
AuthorsHaverty PM, Lin E, Tan J, Yu Y, Lam B, Lianoglou S, Neve RM, Martin S, Settleman J, Yauch RL, Bourgon R
JournalNature
PubMed ID27193678
The use of large-scale genomic and drug response screening of cancer cell lines depends crucially on the reproducibility of results. Here we consider two previously published screens, plus a later critique of these studies. Using independent data, we show that consistency is achievable, and provide a systematic description of the ... More
STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection.
AuthorsQueval CJ, Song OR, Deboosère N, Delorme V, Debrie AS, Iantomasi R, Veyron-Churlet R, Jouny S, Redhage K, Deloison G, Baulard A, Chamaillard M, Locht C, Brodin P
JournalSci Rep
PubMed ID27384401
Mycobacterium tuberculosis is a successful intracellular pathogen. Numerous host innate immune responses signaling pathways are induced upon mycobacterium invasion, however their impact on M. tuberculosis replication is not fully understood. Here we reinvestigate the role of STAT3 specifically inside human macrophages shortly after M. tuberculosis uptake. We first show that ... More
In vitro studies of Norwegian Red bovine semen immobilized and cryopreserved in alginate solid gel network.
AuthorsAlm-Kristiansen AH, Gaustad ER, Bai G, Standerholen FB, Klinkenberg G, Kommisrud E, Waterhouse KE
JournalReprod Domest Anim
PubMed ID29164721
Development of new semen cryopreservation techniques improving sperm survival and ensuring availability of viable spermatozoa for a prolonged time-period after AI is promising tools to reduce sensitivity of timing of AI and enhance overall fertility. The SpermVital ... More
Quantitative assessment of bacterial growth phase utilizing flow cytometry.
AuthorsKhomtchouk KM, Weglarz M, Bekale LA, Koliesnik I, Bollyky PL, Santa Maria PL
JournalJ Microbiol Methods
PubMed ID31678132
Flow cytometry is currently underutilized for bacterial phenotyping and standard microbiological techniques do not provide phenotypic information about the state of the bacterial disease. Pseudomonas aeruginosa is a human pathogen of increased importance in public health due to both the ability to cause chronic diseases and the prevalence of functionally ... More