'Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent ... More
Modulation of [Ca2+]i signaling dynamics and metabolism by perinuclear mitochondria in mouse parotid acinar cells.
AuthorsBruce JI, Giovannucci DR, Blinder G, Shuttleworth TJ, Yule DI
JournalJ Biol Chem
PubMed ID14699167
'Parotid acinar cells exhibit rapid cytosolic calcium signals ([Ca2+]i) that initiate in the apical region but rapidly become global in nature. These characteristic [Ca2+]i signals are important for effective fluid secretion, which critically depends on a synchronized activation of spatially separated ion fluxes. Apically restricted [Ca2+]i signals were never observed ... More
An evaluation of the performance of cDNA microarrays for detecting changes in global mRNA expression.
AuthorsYue H, Eastman PS, Wang BB, Minor J, Doctolero MH, Nuttall RL, Stack R, Becker JW, Montgomery JR, Vainer M, Johnston R
JournalNucleic Acids Res
PubMed ID11292855
'The cDNA microarray is one technological approach that has the potential to accurately measure changes in global mRNA expression levels. We report an assessment of an optimized cDNA microarray platform to generate accurate, precise and reliable data consistent with the objective of using microarrays as an acquisition platform to populate ... More
Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry.
AuthorsDay JP, Kell DB, Griffith GW
JournalAppl Environ Microbiol
PubMed ID11772606
'The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or ... More
The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria.
AuthorsGuy R, Liu P, Pennefather P, Crandall I,
JournalMalar J
PubMed ID17617912
'BACKGROUND: Giemsa staining of thick blood smears remains the "gold standard" for detecting malaria. However, this method is not very good for diagnosing low-level infections. A method for the simultaneous staining of Plasmodium-parasitized culture and blood smears for both bright field and fluorescence was developed and its ability to improve ... More
The alpha(1B/D)-adrenoceptor knockout mouse permits isolation of the vascular alpha(1A)-adrenoceptor and elucidates its relationship to the other subtypes.
AuthorsMethven L, McBride M, Wallace GA, McGrath JC,
JournalBr J Pharmacol
PubMed ID19572943
BACKGROUND AND PURPOSE: Mesenteric and carotid arteries from the alpha(1B/D)-adrenoceptor knockout (alpha(1B/D)-KO) were employed to isolate alpha(1A)-adrenoceptor pharmacology and location and to reveal these features in the wild-type (WT) mouse. EXPERIMENTAL APPROACH: Functional pharmacology by wire myography and receptor localization by confocal microscopy, using the fluorescent alpha(1)-adrenoceptor ligand BODIPY FL-Prazosin ... More
Confocal imaging of microglial cell dynamics in hippocampal slice cultures.
AuthorsDailey ME, Waite M
JournalMethods
PubMed ID10356354
Methods are described for imaging the cellular dynamics of microglia in live mammalian brain slice cultures. Brain slices prepared from developing rat hippocampus are cultured for up to 2 weeks by the roller tube or static filter culture technique, stained with one or more fluorescent dyes, and imaged by scanning ... More
Reply to 'Fetal side' of the placenta: Anatomical mis-annotation of carbon particle 'transfer' across the human placenta.
Authors
JournalNat Commun
PubMed ID34862377
Realistic optical cell modeling and diffraction imaging simulation for study of optical and morphological parameters of nucleus.
Authors
JournalOpt Express
PubMed ID26832267
Quantitative analysis and comparison of 3D morphology between viable and apoptotic MCF-7 breast cancer cells and characterization of nuclear fragmentation.
Authors
JournalPLoS One
PubMed ID28886199
LINE-1 protein localization and functional dynamics during the cell cycle.
Authors
JournalElife
PubMed ID29309036
Label-free detection of uptake, accumulation, and translocation of diesel exhaust particles in ex vivo perfused human placenta.
Authors
JournalJ Nanobiotechnology
PubMed ID34001140
Identifying a Deferiprone-Resveratrol Hybrid as an Effective Lipophilic Anti-Plasmodial Agent.
Authors
JournalMolecules
PubMed ID34279413
Inhibitory effect of novel iron chelator, 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) and green tea extract on growth of Plasmodium falciparum.
Authors
JournalMalar J
PubMed ID26424148
Chromosome choreography during the non-binary cell cycle of a predatory bacterium.
Authors
JournalCurr Biol
PubMed ID34256020
Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity.
Authors
JournalImmunity
PubMed ID30709742
Continuous focal translation enhances rate of point-scan volumetric microscopy.