Nucleic acid binding agents exert local toxic effects on neurites via a non-nuclear mechanism.
AuthorsPin S, Chen H, Lein PJ, Wang MM
JournalJ Neurochem
PubMed ID16441515
'The mechanism by which drugs that target nucleic acids cause neurotoxicity is not well described. We characterized the neurotoxicity of Hoechst 33342 (bis-benzimide), a common cell permeable nuclear dye, in primary neuronal cultures. The mechanism of cell death was not apoptotic, as death is rapid, not accompanied by typical nuclear ... More
Multiparameter detection of apoptosis using red-excitable SYTO probes.
'Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent ... More
Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry.
AuthorsDay JP, Kell DB, Griffith GW
JournalAppl Environ Microbiol
PubMed ID11772606
'The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or ... More
Glutamate release promotes growth of malignant gliomas.
AuthorsTakano T, Lin JH, Arcuino G, Gao Q, Yang J, Nedergaard M
JournalNat Med
PubMed ID11533703
'Glutamate neurotoxicity has been implicated in stroke, head trauma, multiple sclerosis and neurodegenerative diseases. Although recent data show that cultured glioma cells secrete glutamate, the growth potential of brain tumors has not yet been linked to an excitotoxic mechanism. Using bioluminescence detection of glutamate release from freshly prepared brain slices, ... More
Surface imaging microscopy, an automated method for visualizing whole embryo samples in three dimensions at high resolution.
AuthorsEwald AJ, McBride H, Reddington M, Fraser SE, Kerschmann R
JournalDev Dyn
PubMed ID12412023
Modern biology is faced with the challenge of understanding the specification, generation, and maintenance of structures ranging from cells and tissues to organs and organisms. By acquiring images directly from the block face of an embedded sample, surface imaging microscopy (SIM) generates high-resolution volumetric images of biological specimens across all ... More
Variability and memory of protein levels in human cells.
AuthorsSigal A, Milo R, Cohen A, Geva-Zatorsky N, Klein Y, Liron Y, Rosenfeld N, Danon T, Perzov N, Alon U
JournalNature
PubMed ID17122776
Protein expression is a stochastic process that leads to phenotypic variation among cells. The cell-cell distribution of protein levels in microorganisms has been well characterized but little is known about such variability in human cells. Here, we studied the variability of protein levels in human cells, as well as the ... More
Quorum sensing and motility mediate interactions between Pseudomonas aeruginosa and Agrobacterium tumefaciens in biofilm cocultures.
AuthorsAn D, Danhorn T, Fuqua C, Parsek MR
JournalProc Natl Acad Sci U S A
PubMed ID16537456
In the environment, multiple microbial taxa typically coexist as communities, competing for resources and, often, physically associated within biofilms. A dual-species cocultivation model has been developed by using two ubiquitous and well studied microbes Pseudomonas aeruginosa (P.a.) and Agrobacterium tumefaciens (A.t.) as a tractable system to identify molecular mechanisms that ... More
Pseudomonas aeruginosa aggregates in cystic fibrosis sputum produce exopolysaccharides that likely impede current therapies.
Authors
JournalCell Rep
PubMed ID33626358
Spatial transcriptomic and single-nucleus analysis reveals heterogeneity in a gigantic single-celled syncytium.
Authors
JournalElife
PubMed ID35195068
Revealing microbial recognition by specific antibodies.
Authors
JournalBMC Microbiol
PubMed ID26134992
Pyroptosis Induction and Detection.
Authors
JournalCurr Protoc Immunol
PubMed ID30028908
Neutrophil Swarming in Damaged Tissue Is Orchestrated by Connexins and Cooperative Calcium Alarm Signals.
Authors
JournalCurr Biol
PubMed ID32502410
Direct squencing from the minimal number of DNA molecules needed to fill a 454 picotiterplate.
Authors
JournalPLoS One
PubMed ID24887077
Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine.
Authors
JournalCurr Protoc
PubMed ID34004049
Active and Secretory IgA-Coated Bacterial Fractions Elucidate Dysbiosis in Clostridium difficile Infection.
Authors
JournalmSphere
PubMed ID27303742
Active and secreted IgA-coated bacterial fractions from the human gut reveal an under-represented microbiota core.
Authors
JournalSci Rep
PubMed ID24343271
Francisella targets cholesterol-rich host cell membrane domains for entry into macrophages.