SYTO™ 64 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Citations & References (8)

Invitrogen™

SYTO™ 64 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

The cell-permeant SYTO 64 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because theRead more

Catalog Number	Quantity
S11346	100 μL

Catalog number S11346

Price (CNY)

4,794.00

Add to cart

Quantity:

100 μL

Price (CNY)

4,794.00

Add to cart

The cell-permeant SYTO 64 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (Cat. No. S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ColorRed

DescriptionSYTO™ 64 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Detection MethodFluorescence

Dye TypeCell-Permeant

Emission619 nm

Excitation Wavelength Range599 nm

For Use With (Equipment)Fluorescence Microscope

Product LineSYTO

Quantity100 μL

Shipping ConditionRoom Temperature

Volume (Metric)100 μL

Label TypeFluorescent Dye

Product TypeNucleic Acid Stain

SubCellular LocalizationNucleic Acids

Unit SizeEach

Contents & Storage

Store in freezer at -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (8)

Citations & References

Abstract

Exercise training attenuates coronary smooth muscle phenotypic modulation and nuclear Ca2+ signaling.

Authors:Wamhoff BR, Bowles DK, Dietz NJ, Hu Q, Sturek M

Journal:Am J Physiol Heart Circ Physiol

PubMed ID:12388302

'Physical inactivity is an independent risk factor for coronary heart disease, yet the mechanism(s) of exercise-related cardioprotection remains unknown. We tested the hypothesis that coronary smooth muscle after exercise training would have decreased mitogen-induced phenotypic modulation and enhanced regulation of nuclear Ca(2+). Yucatan swine were endurance exercise trained (EX) on ... More

Phosphatidylserine-dependent engulfment by macrophages of nuclei from erythroid precursor cells.

Authors:Yoshida H, Kawane K, Koike M, Mori Y, Uchiyama Y, Nagata S

Journal:Nature

PubMed ID:16193055

'Definitive erythropoiesis usually occurs in the bone marrow or fetal liver, where erythroblasts are associated with a central macrophage in anatomical units called ''blood islands''. Late in erythropoiesis, nuclei are expelled from the erythroid precursor cells and engulfed by the macrophages in the blood island. Here we show that the ... More

Multiparameter detection of apoptosis using red-excitable SYTO probes.

Authors:Wlodkowic D, Skommer J, Hillier C, Darzynkiewicz Z,

Journal:Cytometry A

PubMed ID:18431792

'Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent ... More

Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2.

Authors:Xiao W, Warrington KH, Hearing P, Hughes J, Muzyczka N

Journal:J Virol

PubMed ID:12388712

'We examined cytoplasmic trafficking and nuclear translocation of adeno-associated virus type 2 (AAV) by using Alexa Fluor 488-conjugated wild-type AAV, A20 monoclonal antibody immunocytochemistry, and subcellular fractionation techniques followed by DNA hybridization. Our results indicated that in the absence of adenovirus (Ad), AAV enters the cell rapidly and escapes from ... More

Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry.

Authors:Day JP, Kell DB, Griffith GW

Journal:Appl Environ Microbiol

PubMed ID:11772606

'The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or ... More

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