SYTO™ 40 蓝色荧光核酸染色剂 - 5 mM 的 DMSO 溶液

The cell-permeant SYTO 40 blue fluorescent nucleic acid stain exhibits bright, blue fluorescence upon binding to nucleic acids. Because the了解更多信息

货号	数量
S11351	250 μL

货号 S11351

价格（CNY)

4,757.00

添加至购物车

250 μL

价格（CNY)

4,757.00

添加至购物车

The cell-permeant SYTO 40 blue fluorescent nucleic acid stain exhibits bright, blue fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary from one cell type to another, we offer the SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit (Cat. No. S-11350) to enable researchers to find the dye that works best for a particular application.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

仅供科研使用。不可用于诊断程序。

颜色红色、蓝色

描述SYTO™ 40 蓝色荧光核酸染色剂 - 5 mM 的 DMSO 溶液

检测方法荧光

染料类型细胞通透性

发射441 nm

激发波长范围420 nm

适用于（设备）荧光显微镜

产品线SYTO

数量250 μL

运输条件室温

容积（公制）250 μL

标签类型荧光

产品类型核酸染色剂

亚细胞定位核酸

Unit SizeEach

内容与储存

在冷柜（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

这些染料如何与DNA结合？

SYTO核酸染料的结合方式尚不清楚。但是，SYTO以及相关的核酸染料具有以下结合特性：

1.它们与一些溶剂结合（通过对盐、二价阳离子的敏感性，特别是SDS），因此，它们可能结合到DNA沟槽处。
2.所有SYTO染料都表现出一些碱基选择性，因此，它们可能结合到DNA小沟处。
3.通过乙醇沉淀可从核酸中去除SYTO染料；溴化乙锭和其他嵌入剂不具有此特性。同样，丁醇和氯仿提取不能从核酸中去除SYTO染料，但能够去除溴化乙锭。
4.SYTO 结合不受非离子去垢剂的影响。
5.SYTO染料不会被BrdU淬灭，因此，SYTO染料与核酸的结合方式不同于Hoechst 33342和DAPI（4',6-二脒基-2-苯基吲哚）。

SYBR Green I对移码指示菌几乎没有致突变性，证明其不大可能是强嵌入剂。

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.