Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature.
AuthorsGudnason H,Dufva M,Bang DD,Wolff A
JournalNucleic acids research
PubMed ID17897966
The importance of real-time polymerase chain reaction (PCR) has increased steadily in clinical applications over the last decade. Many applications utilize SYBR Green I dye to follow the accumulation of amplicons in real time. SYBR Green I has, however, a number of limitations that include the inhibition of PCR, preferential ... More
Imaging Poliovirus Entry in Live Cells.
AuthorsBrandenburg B, Lee LY, Lakadamyali M, Rust MJ, Zhuang X, Hogle JM
JournalPLoS Biol
PubMed ID17622193
'Viruses initiate infection by transferring their genetic material across a cellular membrane and into the appropriate compartment of the cell. The mechanisms by which animal viruses, especially nonenveloped viruses, deliver their genomes are only poorly understood. This is due in part to technical difficulties involved in direct visualization of viral ... More
Mitotic spindle rotation and mode of cell division in the developing telencephalon.
AuthorsHaydar TF, Ang E, Rakic P
JournalProc Natl Acad Sci U S A
PubMed ID12589023
'The mode of neural stem cell division in the forebrain proliferative zones profoundly influences neocortical growth by regulating the number and diversity of neurons and glia. Long-term time-lapse multiphoton microscopy of embryonic mouse cortex reveals new details of the complex three-dimensional rotation and oscillation of the mitotic spindle before stem ... More
A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye.
AuthorsWatts MR, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R,
Journal
PubMed ID24323513
'An assay to detect Strongyloides stercoralis in stool specimens was developed using the loop-mediated isothermal amplification (LAMP) method. Primers were based on the 28S ribosomal subunit gene. The reaction conditions were optimized and SYTO-82 fluorescent dye was used to allow real-time and visual detection of the product. The product identity ... More
High-resolution melting curve analysis, a rapid and affordable method for mutation analysis in childhood acute myeloid leukemia.
AuthorsLiu Y, Tang J, Wakamatsu P, Xue H, Chen J, Gaynon PS, Shen S, Sun W,
Journal
PubMed ID25250304
'Molecular genetic alterations with prognostic significance have been described in childhood acute myeloid leukemia (AML). The aim of this study was to establish cost-effective techniques to detect mutations of FMS-like tyrosine kinase 3 (FLT3), nucleophosmin 1 (NPM1), and a partial tandem duplication within the mixed-lineage leukemia (MLL-PTD) genes in childhood ... More
Punctuated Distribution of Recombination Hotspots and Demarcation of Pericentromeric Regions in Phaseolus vulgaris L.
AuthorsBhakta MS, Jones VA, Vallejos CE,
Journal
PubMed ID25629314
'High density genetic maps are a reliable tool for genetic dissection of complex plant traits. Mapping resolution is often hampered by the variable crossover and non-crossover events occurring across the genome, with pericentromeric regions (pCENR) showing highly suppressed recombination rates. The efficiency of linkage mapping can further be improved by ... More
No evidence of conpopulation sperm precedence between allopatric populations of house mice.
AuthorsFirman RC, Simmons LW,
Journal
PubMed ID25295521
Investigations into the evolution of reproductive barriers have traditionally focused on closely related species, and the prevalence of conspecific sperm precedence. The effectiveness of conspecific sperm precedence at limiting gene exchange between species suggests that gametic isolation is an important component of reproductive isolation. However, there is a paucity of ... More
Efficient delivery of antibody into living cells using a novel HVJ envelope vector system.
AuthorsKondo Y, Fushikida K, Fujieda T, Sakai K, Miyata K, Kato F, Kato M,
JournalJ Immunol Methods
PubMed ID18221753
Introduction of antibodies which retain their function into cells using a simple and easy method would be very useful for study of the intracellular events in living cells. In this study, we developed a new method for intracellular delivery of antibody. It includes a combination of a novel IgG-capturing protein ... More
Engineering surfaces for substrate-mediated gene delivery using recombinant proteins.
AuthorsRea JC, Gibly RF, Davis NE, Barron AE, Shea LD,
JournalBiomacromolecules
PubMed ID19775146
Immobilized fibronectin and other natural proteins have been utilized to enhance substrate-mediated gene delivery, with apparent contributions from the intrinsic bioactivity and also physical properties of the immobilized proteins. In this report, we investigated the use of recombinant proteins, compared to the full-length fibronectin protein, as surface coatings for gene ... More
MFP1 is a thylakoid-associated, nucleoid-binding protein with a coiled-coil structure.
AuthorsJeong SY, Rose A, Meier I
JournalNucleic Acids Res
PubMed ID12930969
Plastid DNA, like bacterial and mitochondrial DNA, is organized into protein-DNA complexes called nucleoids. Plastid nucleoids are believed to be associated with the inner envelope in developing plastids and the thylakoid membranes in mature chloroplasts, but the mechanism for this re-localization is unknown. Here, we present the further characterization of ... More
A domain unique to plant RanGAP is responsible for its targeting to the plant nuclear rim.
AuthorsRose A, Meier I
JournalProc Natl Acad Sci U S A
PubMed ID11752475
Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. ... More
Short Periods of Hypoxia Upregulate Sphingosine Kinase 1 and Increase Vasodilation of Arteries to Sphingosine 1-Phosphate (S1P) via S1P
AuthorsAlganga H, Almabrouk TAM, Katwan OJ, Daly CJ, Pyne S, Pyne NJ, Kennedy S
JournalJ Pharmacol Exp Ther
PubMed ID31371480
'Sphingosine kinase [(SK), isoforms SK1 and SK2] catalyzes the formation of the bioactive lipid, sphingosine 1-phosphate (S1P). This can be exported from cells and bind to S1P receptors to modulate vascular function. We investigated the effect of short-term hypoxia on SK1 expression and the response of arteries to S1P. SK1 ... More
Thirty-minute screening of antibiotic resistance genes in bacterial isolates with minimal sample preparation in static self-dispensing 64 and 384 assay cards.
Authors
JournalAppl Microbiol Biotechnol
PubMed ID26227406
Systematic detection of m6A-modified transcripts at single-molecule and single-cell resolution.
Authors
JournalCell Rep Methods
PubMed ID34734208
Surveillance for Western Equine Encephalitis, St. Louis Encephalitis, and West Nile Viruses Using Reverse Transcription Loop-Mediated Isothermal Amplification.
Authors
JournalPLoS One
PubMed ID26807734
Single-tube collection and nucleic acid analysis of clinical samples for SARS-CoV-2 saliva testing.
Authors
JournalSci Rep
PubMed ID35273232
Single-copy detection of somatic variants from solid and liquid biopsy.
Authors
JournalSci Rep
PubMed ID33727644
The Na,K-ATPase-Dependent Src Kinase Signaling Changes with Mesenteric Artery Diameter.
Authors
JournalInt J Mol Sci
PubMed ID30142894
Raman image-activated cell sorting.
Authors
JournalNat Commun
PubMed ID32651381
Targeted Degradation of a Hypoxia-Associated Non-coding RNA Enhances the Selectivity of a Small Molecule Interacting with RNA.
AuthorsCostales MG, Suresh B, Vishnu K, Disney MD
JournalCell Chem Biol
PubMed ID31130520
Small-molecule targeted recruitment of nucleases to RNA is a powerful method to affect RNA biology. Inforna, a sequence-based design approach to target RNA, enables the design of small molecules that bind to and cleave RNA in a selective and substoichiometric manner. Here, we investigate the ability of RNA-targeted degradation to ... More
Glycomics Microarrays Reveal Differential In Situ Presentation of the Biofilm Polysaccharide Poly-
AuthorsFlannery A, Le Berre M, Pier GB, O'Gara JP, Kilcoyne M
JournalInt J Mol Sci
PubMed ID32252300
The biofilm component poly-
Bioorthogonal Metabolic Labeling Utilizing Protein Biosynthesis for Dynamic Visualization of Nonenveloped Enterovirus 71 Infection.
AuthorsWang F, Pan H, Yao X, He H, Liu L, Luo Y, Zhou H, Zheng M, Zhang R, Ma Y, Cai L
JournalACS Appl Mater Interfaces
PubMed ID31845579
Bioorthogonal metabolic labeling through the endogenous cellular metabolic pathways (e.g., phospholipid and sugar) is a promising approach for effectively labeling live viruses. However, it remains a big challenge to label nonenveloped viruses due to lack of host-derived envelopes. Herein, a novel bioorthogonal labeling strategy is developed utilizing protein synthesis pathway ... More