SYTO™ 83 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Citations & References (13)

Invitrogen™

SYTO™ 83 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

The cell-permeant SYTO 83 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because theRead more

Catalog Number	Quantity
S11364	250 μL

Catalog number S11364

Price (CNY)

4,794.00

Add to cart

Quantity:

250 μL

Price (CNY)

4,794.00

Add to cart

The cell-permeant SYTO 83 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (Cat. No. S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ColorOrange

DescriptionSYTO™ 83 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Detection MethodFluorescence

Dye TypeCell-Permeant

Emission559 nm

Excitation Wavelength Range543 nm

For Use With (Equipment)Fluorescence Microscope

Product LineSYTO

Quantity250 μL

Shipping ConditionRoom Temperature

Volume (Metric)250 μL

Label TypeFluorescent Dye

Product TypeNucleic Acid Stain

SubCellular LocalizationNucleic Acids

Unit SizeEach

Contents & Storage

Store in freezer at -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (13)

Citations & References

Abstract

Antibody to a conserved antigenic target is protective against diverse prokaryotic and eukaryotic pathogens.

Authors:Cywes-Bentley C, Skurnik D, Zaidi T, Roux D, Deoliveira RB, Garrett WS, Lu X, O'Malley J, Kinzel K, Zaidi T, Rey A, Perrin C, Fichorova RN, Kayatani AK, Maira-Litràn T, Gening ML, Tsvetkov YE, Nifantiev NE, Bakaletz LO, Pelton SI, Golenbock DT, Pier GB,

Journal:

PubMed ID:23716675

'Microbial capsular antigens are effective vaccines but are chemically and immunologically diverse, resulting in a major barrier to their use against multiple pathogens. A ß-(1?6)-linked poly-N-acetyl-d-glucosamine (PNAG) surface capsule is synthesized by four proteins encoded in genetic loci designated intercellular adhesion in Staphylococcus aureus or polyglucosamine in selected Gram-negative bacterial ... More

Antibodies to a novel leptospiral protein, LruC, in the eye fluids and sera of horses with Leptospira-associated uveitis.

Authors:Verma A, Matsunaga J, Artiushin S, Pinne M, Houwers DJ, Haake DA, Stevenson B, Timoney JF,

Journal:Clin Vaccine Immunol

PubMed ID:22237897

Screening of an expression library of Leptospira interrogans with eye fluids from uveitic horses resulted in identification of a novel protein, LruC. LruC is located in the inner leaflet of the leptospiral outer membrane, and an lruC gene was detected in all tested pathogenic L. interrogans strains. LruC-specific antibody levels ... More

Structural and functional characterizations of SsgB, a conserved activator of developmental cell division in morphologically complex actinomycetes.

Authors:Xu Q, Traag BA, Willemse J, McMullan D, Miller MD, Elsliger MA, Abdubek P, Astakhova T, Axelrod HL, Bakolitsa C, Carlton D, Chen C, Chiu HJ, Chruszcz M, Clayton T, Das D, Deller MC, Duan L, Ellrott K, Ernst D, Farr CL, Feuerhelm J, Grant JC, Grzechnik A, Grzechnik SK, Han GW, Jaroszewski L, Jin KK, Klock HE, Knuth MW, Kozbial P, Krishna SS, Kumar A, Marciano D, Minor W, Mommaas AM, Morse AT, Nigoghossian E, Nopakun A, Okach L, Oommachen S, Paulsen J, Puckett C, Reyes R, Rife CL, Sefcovic N, Tien

Journal:J Biol Chem

PubMed ID:19567872

SsgA-like proteins (SALPs) are a family of homologous cell division-related proteins that occur exclusively in morphologically complex actinomycetes. We show that SsgB, a subfamily of SALPs, is the archetypal SALP that is functionally conserved in all sporulating actinomycetes. Sporulation-specific cell division of Streptomyces coelicolor ssgB mutants is restored by introduction ... More

Perivascular cells with pericyte characteristics are involved in ATP- and PGE(2)-induced relaxation of porcine retinal arterioles in vitro.

Authors:Misfeldt MW, Pedersen SM, Bek T,

Journal:Invest Ophthalmol Vis Sci

PubMed ID:23599323

Relaxation of porcine retinal arterioles in vitro has been shown to be preceded by calcium activity in a population of perivascular cells that cannot be classified as neurons, glial cells, or vascular smooth muscle cells. The purpose of the present investigation was to study calcium activity in these perivascular cells ... More

The use of carbohydrate microarrays to study carbohydrate-cell interactions and to detect pathogens.

Authors:Disney MD, Seeberger PH,

Journal:Chem Biol

PubMed ID:15610854

The use of carbohydrate microarrays to investigate the carbohydrate binding specificities of bacteria, to detect pathogens, and to screen antiadhesion therapeutics is reported. This system is ideal for whole-cell applications because microarrays present carbohydrate ligands in a manner that mimics interactions at cell-cell interfaces. Other advantages include assay miniaturization, since ... More

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