所有SYBR染料都具有相似的光谱性质,但化学组成不同。所有SYBR染料都与dsDNA、ssDNA和RNA结合,但敏感性不同。SYBRSafe DNA凝胶染料是专门研发的溴化乙锭安全替代品。SYBR Green I是dsDNA的超灵敏染料,而SYBR Green II是RNA和ssDNA的高灵敏性染料。所有SYBR染料都适用于Safe Imager蓝光透射仪激发观察。
The transcriptional coactivator CREB-binding protein cooperates with STAT1 and NF-kappa B for synergistic transcriptional activation of the CXC ligand 9/monokine induced by interferon-gamma gene.
Authors:Hiroi M, Ohmori Y
Journal:J Biol Chem
PubMed ID:12403783
'Signal transducers and activators of transcription 1 (STAT1) and NF-kappaB cooperatively regulate the expression of many inflammatory genes. In the present study, we demonstrate that the transcriptional coactivator CREB-binding protein (CBP) mediated the STAT1/NF-kappaB synergy for transcription of the gene for CXC ligand 9 (CXCL9), an interferon-gamma (IFN-gamma)-inducible chemokine. Reporter ... More
Gel staining methods for detection of telomerase activity with the telomeric repeat amplification protocol (TRAP) assay.
Authors:Fujita M, Tomita S, Ueda Y, Fujimori T
Journal:Mol Pathol
PubMed ID:10193516
DOTAP cationic liposomes prefer relaxed over supercoiled plasmids.
Authors:Even-Chen S, Barenholz Y
Journal:Biochim Biophys Acta
PubMed ID:11118529
'Cationic liposomes and DNA interact electrostatically to form complexes called lipoplexes. The amounts of unbound (free) DNA in a mixture of cationic liposomes and DNA at different cationic lipid:DNA molar ratios can be used to describe DNA binding isotherms; these provide a measure of the binding efficiency of DNA to ... More
Application of digital image analysis and flow cytometry to enumerate marine viruses stained with SYBR gold.
Authors:Chen F, Lu JR, Binder BJ, Liu YC, Hodson RE
Journal:Appl Environ Microbiol
PubMed ID:11157214
'A novel nucleic acid stain, SYBR Gold, was used to stain marine viral particles in various types of samples. Viral particles stained with SYBR Gold yielded bright and stable fluorescent signals that could be detected by a cooled charge-coupled device camera or by flow cytometry. The fluorescent signal strength of ... More