Laser capture microdissection and protein microarray analysis of human non-small cell lung cancer: differential epidermal growth factor receptor (EGPR) phosphorylation events associated with mutated EGFR compared with wild type.
AuthorsVanMeter AJ, Rodriguez AS, Bowman ED, Jen J, Harris CC, Deng J, Calvert VS, Silvestri A, Fredolini C, Chandhoke V, Petricoin EF, Liotta LA, Espina V,
JournalMol Cell Proteomics
PubMed ID18687633
'Little is known about lung carcinoma epidermal growth factor (EGF) kinase pathway signaling within the context of the tissue microenvironment. We quantitatively profiled the phosphorylation and abundance of signal pathway proteins relevant to the EGF receptor within laser capture microdissected untreated, human non-small cell lung cancer (NSCLC) (n = 25) ... More
Novel observations of Thiobacterium, a sulfur-storing Gammaproteobacterium producing gelatinous mats.
AuthorsGrünke S, Lichtschlag A, de Beer D, Kuypers M, Lösekann-Behrens T, Ramette A, Boetius A,
JournalISME J
PubMed ID20220790
The genus Thiobacterium includes uncultivated rod-shaped microbes containing several spherical grains of elemental sulfur and forming conspicuous gelatinous mats. Owing to the fragility of mats and cells, their 16S ribosomal RNA genes have not been phylogenetically classified. This study examined the occurrence of Thiobacterium mats in three different sulfidic marine ... More
Herp, a new ubiquitin-like membrane protein induced by endoplasmic reticulum stress.
AuthorsKokame K, Agarwala KL, Kato H, Miyata T
JournalJ Biol Chem
PubMed ID10922362
Hyperhomocysteinemia, a risk factor for vascular disease, injures endothelial cells through undefined mechanisms. We previously identified several homocysteine-responsive genes in cultured human vascular endothelial cells, including the endoplasmic reticulum (ER)-resident molecular chaperone GRP78/BiP. Here, we demonstrate that homocysteine induces the ER stress response and leads to the expression of a ... More
TrkB has a cell-autonomous role in the establishment of hippocampal Schaffer collateral synapses.
AuthorsLuikart BW, Nef S, Virmani T, Lush ME, Liu Y, Kavalali ET, Parada LF
JournalJ Neurosci
PubMed ID15829629
Neurotrophin signaling has been implicated in the processes of synapse formation and plasticity. To gain additional insight into the mechanism of BDNF and TrkB influence on synapse formation and synaptic plasticity, we generated a conditional knock-out for TrkB using the cre/loxp system. Using three different cre-expressing transgenic mice, three unique ... More
A thousand points of light: the application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics.
AuthorsPatton WF
JournalElectrophoresis
PubMed ID10786886
As proteomics evolves into a high-throughput technology for the study of global protein regulation, new demands are continually being placed upon protein visualization and quantitation methods. Chief among these are increased detection sensitivity, broad linear dynamic range and compatibility with modern methods of microchemical analyses. The limitations of conventional protein ... More
A luminescent ruthenium complex for ultrasensitive detection of proteins immobilized on membrane supports.
SYPRO Ruby protein blot stain provides a sensitive, gentle, fluorescence-based method for detecting proteins on nitrocellulose or polyvinylidene difluoride (PVDF) membranes. SYPRO Ruby dye is a permanent stain composed of ruthenium as part of an organic complex that interacts noncovalently with proteins. Stained proteins can be excited by ultraviolet light ... More
Making blind robots see: the synergy between fluorescent dyes and imaging devices in automated proteomics.
AuthorsPatton WF
JournalBiotechniques
PubMed ID10818702
Proteomics investigations endeavor to provide a global understanding of gene product synthesis rate, degradation rate, functional competence, posttranslational modification, subcellular distribution and physical interactions with other cell components. Protein expression encompasses an enormous dynamic range. Since rare proteins cannot be amplified by any type of PCR method, sensitive detection is ... More
A general method for the rapid characterization of tyrosine-phosphorylated proteins by mini two-dimensional gel electrophoresis.
AuthorsDucret A, Desponts C, Desmarais S, Gresser MJ, Ramachandran C
JournalElectrophoresis
PubMed ID10892730
Our preliminary results are reported in the investigation of the tyrosine phosphorylation cascade triggered by the stimulation of the insulin receptor in the adipocyte cell line 3T3-L1 using a mini two-dimensional gel electrophoresis approach. The minigel format, 8 x 10 cm, was found sufficiently resolving and reproducible to study complex ... More
Mapping glycosylation changes related to cancer using the Multiplexed Proteomics technology: a protein differential display approach.
AuthorsSchulenberg B, Beechem JM, Patton WF
JournalJ Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID12880860
The metastatic spread of tumor cells in malignant progression is known to be a major cause of cancer mortality. Protein glycosylation is increasingly being recognized as one of the most prominent biochemical alterations associated with malignant transformation and tumorigenesis. The Multiplexed Proteomics (MP) approach is a new technology that permits ... More
The utrophin A 5'-untranslated region confers internal ribosome entry site-mediated translational control during regeneration of skeletal muscle fibers.
AuthorsMiura P, Thompson J, Chakkalakal JV, Holcik M, Jasmin BJ
JournalJ Biol Chem
PubMed ID16061482
Utrophin up-regulation in muscle fibers of Duchenne muscular dystrophy patients represents a potential therapeutic strategy. It is thus important to delineate the regulatory events presiding over utrophin in muscle in attempts to develop pharmacological interventions aimed at increasing utrophin expression. A number of studies have now shown that under several ... More