Reversible inactivation of myosin subfragment 1 activity by mechanical immobilization.
AuthorsHighsmith S, Duignan K, Franks-Skiba K, Polosukhina K, Cooke R
JournalBiophys J
PubMed ID9512042
'The Mg-ATPase activity of skeletal muscle myosin subfragment 1 (S1) is reversibly eliminated when it is aggregated by the force of osmotic pressure dehydration using polyethylene glycol (PEG). Several experiments indicate nucleotides bind aggregated S1, but the effects of binding are attenuated. Compared with S1 in solution, epsilonADP binds aggregated ... More
Covalent attachment of fluorescent probes to the X-base of Escherichia coli phenylalanine transfer ribonucleic acid.
AuthorsSchiller PW, Schechter AN
JournalNucleic Acids Res
PubMed ID333386
tRNA PheE, coli was labeled with the N-hydroxysuccinimide esters of 1-dimethylaminonaphthalene-5-sulfonyl glycine and N-methylanthranilic acid through reaction with the amino acid moiety of its X-base, whereby yields of 66% and 24%, respectively, were obtained. The purified dimethylaminonaphthalene-sulfonate derivative could not be aminoacylated and was found to be a strong competitive ... More
Ca(2+)-dependent fluorescence transients and phosphate metabolism during low-flow ischemia in rat hearts.
AuthorsCamacho SA, Figueredo VM, Brandes R, Weiner MW
JournalAm J Physiol
PubMed ID8342622
To determine whether cytosolic free calcium ([Ca2+]i) rises during low-flow ischemia and to determine the mechanisms responsible for contractile dysfunction, isolated rat hearts were studied during graded reductions of coronary flow. Indo1 fluorescence at 385- and 456-nm wave-lengths (F385/456) was used as an index of [Ca2+]i. 31P-magnetic resonance spectroscopy (MRS) ... More
A high throughput fluorogenic substrate for interstitial collagenase (MMP-1) and gelatinase (MMP-9).
AuthorsBickett DM, Green MD, Berman J, Dezube M, Howe AS, Brown PJ, Roth JT, McGeehan GM
JournalAnal Biochem
PubMed ID8368516
Two members of the matrix metalloproteinase family of enzymes, interstitial collagenase and 92-kDa gelatinase, have been implicated in the pathogenesis of rheumatoid arthritis and tumor metastasis. In order to characterize the activities of these enzymes, we have developed a fluorogenic peptide substrate which is efficiently hydrolyzed by both enzymes. This ... More
Preparation and characterization of fluorescent scorpion toxins from Leiurus quinquestriatus quinquestriatus as probes of the sodium channel of excitable cells.
AuthorsAngelides KJ, Nutter TJ
JournalJ Biol Chem
PubMed ID6311830
Fluorescent derivatives of scorpion toxin V from Leiurus quinquestriatus quinquestriatus have been prepared so that the topographical, dynamic, and cellular properties of the neurotoxin receptor site on the voltage-dependent sodium channel could be studied. Four different modification strategies have been pursued in which acylated, amidinylated, thio-amidinylated, and reductively alkylated scorpion ... More