Mechanism of peptide-induced mast cell degranulation. Translocation and patch-clamp studies.
AuthorsLorenz D, Wiesner B, Zipper J, Winkler A, Krause E, Beyermann M, Lindau M, Bienert M
JournalJ Gen Physiol
PubMed ID9806967
Substance P and other polycationic peptides are thought to stimulate mast cell degranulation via direct activation of G proteins. We investigated the ability of extracellularly applied substance P to translocate into mast cells and the ability of intracellularly applied substance P to stimulate degranulation. In addition, we studied by reverse ... More
Characterization of a fluorescent substance P analog.
AuthorsTota MR, Daniel S, Sirotina A, Mazina KE, Fong TM, Longmore J, Strader CD
JournalBiochemistry
PubMed ID7524660
We describe the development and characterization of substance P labeled at Lys3 with fluorescein ([fluorescein Lys3]SP) as a fluorescent probe for the neurokinin 1 (NK1) receptor. [fluorescein Lys3]SP is an agonist at the human NK1 receptor, with an affinity for both the high-affinity and low-affinity binding states of the receptor ... More