Alexa Fluor™ 633 链霉亲和素偶联物

引用和文献 (5)

Invitrogen™

Alexa Fluor™ 633 链霉亲和素偶联物

Alexa Fluor™ 633 链霉亲和素由生物素结合蛋白（链霉亲和素）与荧光标记物（Alexa Fluor™ 染料）共价结合而成了解更多信息

货号	数量
S21375	1 mg

货号 S21375

价格（CNY)

4,739.00

添加至购物车

价格（CNY)

4,739.00

添加至购物车

Alexa Fluor™ 633 链霉亲和素由生物素结合蛋白（链霉亲和素）与荧光标记物（Alexa Fluor™ 染料）共价结合而成。链霉亲和素与生物素结合的亲和力非常高，带链霉亲和素的偶联物通常和带生物素的偶联物一起使用，专门用于检测各种蛋白、蛋白模体、核酸或其他分子（例如与生物素偶联的一抗结合目标蛋白后，可以用荧光标记的链霉亲和素检测）。与此相似的检测策略在多种检测方法中使用，包括蛋白质免疫印迹、流式细胞分析、成像和显微检测、微孔板检测等。Alexa Fluor™ 染料链霉亲和素偶联物以 1 mg 冻干产品或 0.5 mL 体积的 2 mg/mL 溶液形式提供。

Alexa Fluor™ 633 链霉亲和素偶联物的重要特点：
•Alexa Fluor™ 633 链霉素亲和素偶联物的最大激发/发射波长分别为 ∼ (632/647)
• 光稳定性强荧光
• 在水溶液中的溶解度高
• 多种颜色可选
• 非常适合蛋白质免疫印迹、流式细胞分析、成像和显微镜检查、微孔板测定等应用

Alexa Fluor™ 染料的性质
Alexa Fluor™ 染料是专为提升成像和其他标记方案性能而开发的有机荧光染料，光稳定性和亮度更高，在水溶液中的溶解性更佳。这类染料提供多种颜色选择，是大多数成像应用的理想之选。

封闭内源性生物素
天然生成的生物素可干扰生物素-链霉亲和素检测方案。对于涉及固定或通透化细胞的实验，可使用我们的内源性生物素封闭试剂盒最大限度减少这种干扰。

仅限研究用途。不得用于人或动物的治疗或诊断。

相关链接：

在《Molecular Probes》手册中了解更多有关亲和素-生物素检测的信息

了解更多有关 Alexa Fluor™ 染料的信息

了解其他标记链霉亲和素偶联物

阅读亲和素和链霉亲和素偶联物 - 第7.6节

仅供科研使用。不可用于诊断程序。

标签或染料Alexa Fluor 染料

产品类型链霉素亲和素偶联物(荧光)

数量1 mg

运输条件室温

偶联物Alexa Fluor 633

产品线Alexa Fluor

Unit SizeEach

内容与储存

储存在冰箱(-5 至 -30°C)中并避光。

常见问题解答 (FAQ)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

引用和文献 (5)

引用和文献

Abstract

Development of homogeneous binding assays based on fluorescence resonance energy transfer between quantum dots and Alexa Fluor fluorophores.

Authors:Nikiforov TT, Beechem JM

Journal:Anal Biochem

PubMed ID:16860286

'We studied the fluorescence resonance energy transfer (FRET) between quantum dots emitting at 565, 605, and 655 nm as energy donors and Alexa Fluor fluorophores with absorbance maxima at 594, 633, 647, and 680 nm as energy acceptors. As a first step, we prepared covalent conjugates between all three types ... More

Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library.

Authors:Feldhaus MJ, Siegel RW, Opresko LK, Coleman JR, Feldhaus JM, Yeung YA, Cochran JR, Heinzelman P, Colby D, Swers J, Graff C, Wiley HS, Wittrup KD

Journal:Nat Biotechnol

PubMed ID:12536217

'A nonimmune library of 10(9) human antibody scFv fragments has been cloned and expressed on the surface of yeast, and nanomolar-affinity scFvs routinely obtained by magnetic bead screening and flow-cytometric sorting. The yeast library can be amplified 10(10)-fold without measurable loss of clonal diversity, allowing its effectively indefinite expansion. The ... More

FRET or no FRET: a quantitative comparison.

Authors:Berney C, Danuser G

Journal:Biophys J

PubMed ID:12770904

'Fluorescence resonance energy transfer (FRET) is a technique used to measure the interaction between two molecules labeled with two different fluorophores (the donor and the acceptor) by the transfer of energy from the excited donor to the acceptor. In biological applications, this technique has become popular to qualitatively map protein-protein ... More

Galectin-1, -2, and -3 exhibit differential recognition of sialylated glycans and blood group antigens.

Authors:Stowell SR, Arthur CM, Mehta P, Slanina KA, Blixt O, Leffler H, Smith DF, Cummings RD,

Journal:J Biol Chem

PubMed ID:18216021

Human galectins have functionally divergent roles, although most of the members of the galectin family bind weakly to the simple disaccharide lactose (Galbeta1-4Glc). To assess the specificity of galectin-glycan interactions in more detail, we explored the binding of several important galectins (Gal-1, Gal-2, and Gal-3) using a dose-response approach toward ... More

Normal telomere length and chromosomal end capping in poly(ADP-ribose) polymerase-deficient mice and primary cells despite increased chromosomal instability.

Authors:Samper E, Goytisolo FA, Ménissier-de Murcia J, González-Suárez E, Cigudosa JC, de Murcia G, Blasco MA

Journal:J Cell Biol

PubMed ID:11448989

Poly(ADP-ribose) polymerase (PARP)-1, a detector of single-strand breaks, plays a key role in the cellular response to DNA damage. PARP-1-deficient mice are hypersensitive to genotoxic agents and display genomic instability due to a DNA repair defect in the base excision repair pathway. A previous report suggested that PARP-1-deficient mice also ... More