Streptavidin Acrylamide

Streptavidin acrylamide can be co-polymerized with acrylamide on polymeric surfaces to create a uniform monolayer of immobilized streptavidin. The streptavidin can then be used for biotinylated-probe isolation, utilizing probes, enzymes, antibodies, or pharmaceutical agents. Streptavidin acrylamide can coat polymeric surfaces such as beads or microspheres that range from 1 to 1000 μm in diameter. The flexibility of using streptavidin acrylamide to coat these surfaces allows for complete customization.

Streptavidin-coated microspheres (microparticles) can be used for a variety of affinity binding applications. Streptavidin rapidly binds to biotin with a strong non-covalent bond and high affinity, allowing this amplified bound complex to remain stable within a range of temperature and pH conditions. This makes streptavidin acrylamide coated microspheres a suitable material for pharmaceutical drug delivery, 3D cell culture, bacterial isolation, and flow cytometry analysis. Streptavidin-coated beads are often utilized for DNA sequencing, PCR product cleanup, cell separation, DNA single molecule imaging studies, and in a variety of diagnostic applications.