Anti-biotin antibodies offer superior organelle-specific labeling of mitochondria over avidin or streptavidin.
AuthorsHollinshead M, Sanderson J, Vaux DJ
JournalJ Histochem Cytochem
PubMed ID9267466
The mitochondrial matrix contains endogenously biotinylated proteins. These proteins can cause unexpected background signal when biotin-avidin- or biotin-streptavidin-based detection systems are used in immunocytochemistry. Here we show that this reactivity can be deliberately exploited, using a simple anti-biotin reagent, to obtain strong and highly specific labeling of mitochondria by both ... More