链霉素亲和素，Alexa Fluor™ 750 偶联物

引用和文献 (4)

Invitrogen™

链霉素亲和素，Alexa Fluor™ 750 偶联物

Alexa Fluor™ 750 链霉亲和素由生物素结合蛋白（链霉亲和素）与荧光标记物（Alexa Fluor™ 染料）共价结合而成了解更多信息

货号	数量
S21384	1 mg

货号 S21384

价格（CNY)

4,459.00

添加至购物车

价格（CNY)

4,459.00

添加至购物车

Alexa Fluor™ 750 链霉亲和素由生物素结合蛋白（链霉亲和素）与荧光标记物（Alexa Fluor™ 染料）共价结合而成。链霉亲和素与生物素结合的亲和力非常高，带链霉亲和素的偶联物通常和带生物素的偶联物一起使用，专门用于检测各种蛋白、蛋白模体、核酸或其他分子（例如与生物素偶联的一抗结合目标蛋白后，可以用荧光标记的链霉亲和素检测）。与此相似的检测策略在多种检测方法中使用，包括蛋白质免疫印迹、流式细胞分析、成像和显微检测、微孔板检测等。Alexa Fluor™ 染料链霉亲和素偶联物以 1 mg 冻干产品或 0.5 mL 体积的 2 mg/mL 溶液形式提供。

Alexa Fluor™ 750 链霉亲和素偶联物的重要特点：
•Alexa Fluor™ 750 链霉素亲和素偶联物的最大激发/发射波长分别为 ∼ (749/775)
• 光稳定性强荧光
• 在水溶液中的溶解度高
• 多种颜色可选
• 非常适合蛋白质免疫印迹、流式细胞分析、成像和显微镜检查、微孔板测定等应用

Alexa Fluor™ 染料的性质
Alexa Fluor™ 染料是专为提升成像和其他标记方案性能而开发的有机荧光染料，光稳定性和亮度更高，在水溶液中的溶解性更佳。这类染料提供多种颜色选择，是大多数成像应用的理想之选。

封闭内源性生物素
天然生成的生物素可干扰生物素-链霉亲和素检测方案。对于涉及固定或通透化细胞的实验，可使用我们的内源性生物素封闭试剂盒最大限度减少这种干扰。

仅限研究用途。不得用于人或动物的治疗或诊断。

相关链接：

在《Molecular Probes》手册中了解更多有关亲和素-生物素检测的信息

了解更多有关 Alexa Fluor™ 染料的信息

了解其他标记链霉亲和素偶联物

阅读亲和素和链霉亲和素偶联物 - 第7.6节

仅供科研使用。不可用于诊断程序。

标签或染料Alexa Fluor 染料

产品类型链霉素亲和素偶联物(荧光)

数量1 mg

运输条件室温

偶联物Alexa Fluor 750

产品线Alexa Fluor

Unit SizeEach

内容与储存

在冷冻冰箱(-5 至 -30°C)中避光储存。

常见问题解答 (FAQ)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

引用和文献 (4)

引用和文献

Abstract

In vivo assembly of nanoparticle components to improve targeted cancer imaging.

Authors:Perrault SD, Chan WC,

Journal:Proc Natl Acad Sci U S A

PubMed ID:20534561

Many small molecular anticancer agents are often ineffective at detecting or treating cancer due to their poor pharmacokinetics. Using nanoparticles as carriers can improve this because their large size reduces clearance and improves retention within tumors, but it also slows their rate of transfer from circulation into the tumor interstitium. ... More

DNA replication origin plasticity and perturbed fork progression in human inverted repeats.

Authors:Lebofsky R, Bensimon A

Journal:Mol Cell Biol

PubMed ID:16024811

The stability of metazoan genomes during their duplication depends on the spatiotemporal activation of origins and the progression of forks. Human rRNA genes represent a unique challenge to DNA replication since a large proportion of them exist as noncanonical palindromes in addition to canonical tandem repeats. Whether origin usage and/or ... More

Canonical transient receptor potential 1 plays a role in basic fibroblast growth factor (bFGF)/FGF receptor-1-induced Ca2+ entry and embryonic rat neural stem cell proliferation.

Authors:Fiorio Pla A, Maric D, Brazer SC, Giacobini P, Liu X, Chang YH, Ambudkar IS, Barker JL

Journal:J Neurosci

PubMed ID:15758179

Basic fibroblast growth factor (bFGF) and its major receptor FGF receptor-1 (FGFR-1) play an important role in the development of the cortex. The mechanisms underlying the mitogenic role of bFGF/FGFR-1 signaling have not been elucidated. Intracellular Ca2+ concentrations ([Ca2+]i) in proliferating cortical neuroepithelial cells are markedly dependent on Ca2+ entry ... More

Self-renewing and differentiating properties of cortical neural stem cells are selectively regulated by basic fibroblast growth factor (FGF) signaling via specific FGF receptors.

Authors:Maric D, Fiorio Pla A, Chang YH, Barker JL

Journal:J Neurosci

PubMed ID:17314281

Developmental processes mediating the initiation of lineage commitment from self-renewing neural stem cells (NSCs) remain mostly unclear because of the persisting ambiguity in identifying true NSCs from proliferative lineage-restricted progenitors (LRPs), which are directly or indirectly derived from NSCs. Our multilineage immunohistochemical analyses of early embryonic rat telencephalon at the ... More