Immunological effects of transgenic constitutive expression of the type 1 sphingosine 1-phosphate receptor by mouse lymphocytes.
AuthorsGräler MH, Huang MC, Watson S, Goetzl EJ
JournalJ Immunol
PubMed ID15699128
'The type 1 sphingosine 1-phosphate (S1P) G protein-coupled receptor (S1P1) normally transduces S1P effects on lymph node (LN) egress and tissue migration of naive lymphocytes. We now show that persistent expression of S1P1 by lymphocytes of S1P1-transgenic (Tg) mice suppresses delayed-type hypersensitivity and results in production of significantly more IgE ... More
Discrepancy in measuring CD4 expression on T-lymphocytes using fluorescein conjugates in comparison with unimolar CD4-phycoerythrin conjugates.
'BACKGROUND: Numerous methods for quantitative fluorescence calibration (QFC) have been developed to quantify receptor expression on lymphocytes. However, the results from the use of these different QFC methods vary considerably in the literature. To better identify the causes of these discrepancies, we measured CD4 expression using FITC and phycoerythrin (PE) ... More
Tracking cell proliferation using the far red fluorescent dye SNARF-1.
AuthorsMagg T, Albert MH,
JournalCytometry B Clin Cytom
PubMed ID17397063
BACKGROUND: The [(3)H]thymidine incorporation assay and staining of living cells with fluorescent dyes like carboxyfluorescein diacetate, succinimidyl ester (CFSE) have evolved as valuable methods for studying T cell responses. To assess proliferation of cells already labeled by FITC, CFSE, GFP, or other ... More
Dynamics of thymocyte-stromal cell interactions visualized by two-photon microscopy.
AuthorsBousso P, Bhakta NR, Lewis RS, Robey E
JournalScience
PubMed ID12052962
Thymocytes are selected to mature according to their ability to interact with self major histocompatibility complex (MHC)-peptide complexes displayed on the thymic stroma. Using two-photon microscopy, we performed real-time analysis of the cellular contacts made by developing thymocytes undergoing positive selection in a three-dimensional thymic organ culture. A large fraction ... More
The relationship between Na(+)/H(+) exchanger expression and tyrosinase activity in human melanocytes.
The activity of melanosome-associated tyrosinase in human melanocytes differs based on racial skin type. In melanocytes from Black skin, tyrosinase activity is high while in White melanocytes the activity of the enzyme is low. Recent studies suggest that low tyrosinase activity in White melanocytes may be due to an acidic ... More
Suppression of glomerulonephritis in NZB/NZW lupus prone mice by adoptive transfer of ex vivo expanded regulatory T cells.
Authors
JournalPLoS One
PubMed ID19551149
Probing the luminal microenvironment of reconstituted epithelial microtissues.
Authors
JournalSci Rep
PubMed ID27619235
Limited utility of acetoxymethyl (AM)-based intracellular delivery systems, in vivo: interference by extracellular esterases.
Authors
JournalJ Microsc
PubMed ID17381712
Dynamic imaging of the effector immune response to listeria infection in vivo.
Authors
JournalPLoS Pathog
PubMed ID21455492
Computational spatiotemporal analysis identifies WAVE2 and cofilin as joint regulators of costimulation-mediated T cell actin dynamics.
Authors
JournalSci Signal
PubMed ID27095595
Amplifying fluorescence signal contrast of aptamer-modified microspheres inspired by whispering-gallery mode lasing.
Authors
JournalRSC Adv
PubMed ID35542363
A Reliable and Non-destructive Method for Monitoring the Stromal pH in Isolated Chloroplasts Using a Fluorescent pH Probe.
Authors
JournalFront Plant Sci
PubMed ID29259618
Mimicking Angiogenesis in vitro: Three-dimensional Co-culture of Vascular Endothelial Cells and Perivascular Cells in Collagen Type I Gels.