SAIVI™ 快速抗体标记试剂盒，Alexa Fluor™ 680
SAIVI™ 快速抗体标记试剂盒，Alexa Fluor™ 680
引用和文献 (5)
Invitrogen™

SAIVI™ 快速抗体标记试剂盒，Alexa Fluor™ 680

Alexa Fluor™ 680 SAIVI™ 抗体标记试剂盒提供了一种标记抗体的便利方法，在 6 倍抗体浓度范围内具有适用于体内成像应用的较优标记度 (DOL; ∼2)，无需调整反应体积、染料浓度或抗体浓度了解更多信息
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货号数量
S300451 kit
货号 S30045
价格（CNY)
13,263.00
Each
添加至购物车
数量:
1 kit
价格（CNY)
13,263.00
Each
添加至购物车
Alexa Fluor™ 680 SAIVI™ 抗体标记试剂盒提供了一种标记抗体的便利方法，在 6 倍抗体浓度范围内具有适用于体内成像应用的较优标记度 (DOL; ∼2)，无需调整反应体积、染料浓度或抗体浓度。使用该程序，较佳标记抗体就可以直接用于需要不含叠氮化物试剂的应用，例如活细胞成像或直接注射至动物。
仅供科研使用。不可用于诊断程序。
规格
颜色红外
检测方法荧光
激发/发射679/702
标签类型Alexa Fluor 染料
标记规模0.5 至 3 mg
产品线Alexa Fluor、SAIVI
产品类型抗体标记试剂盒
数量1 kit
运输条件室温
标记目标抗体
标签或染料Alexa Fluor 680
Unit SizeEach
内容与储存
在冷藏冰箱（2°C 至 8°C）中避光储存。

常见问题解答 (FAQ)

我该注射多少量的偶联抗体来进行肿瘤成像？

推荐的起始剂量为50 µg。您需要根据实验模型来确定最佳剂量。

我应该采用哪种规格的针头？

我们建议使用具有固定（不可移动）针头、28-32管径的结核菌素和胰岛素注射器（0.3或1.0 mL容量）。

能够注入小鼠体内的最佳和最大试剂体积是多少？

注射试剂的体积根据给药途径而异。下面是25克动物的一般指导用量：

静脉注射（IV）50–125 µL（推荐体积）- 200 µL（最大体积）；腹腔注射（IP）500 µL（推荐体积）- 2 mL（最大体积）；皮下注射（SC）100–250 µL（推荐体积）- 1 mL（最大体积）。

哪种类型的小鼠最适于体内成像？

由于毛皮会引起光散射，所以推荐采用无毛小鼠（如无胸腺裸鼠（nu/nu））进行体内成像。如果无法使用裸鼠，覆盖成像区域的毛发应该用剃毛机或化学脱毛剂（如Nair脱毛剂）去除。

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all SAIVI™ 快速抗体标记试剂盒，Alexa Fluor™ 680 FAQs

引用和文献 (5)

引用和文献
Abstract
Affibody Molecules for In vivo Characterization of HER2-Positive Tumors by Near-Infrared Imaging.
Authors:Lee SB, Hassan M, Fisher R, Chertov O, Chernomordik V, Kramer-Marek G, Gandjbakhche A, Capala J,
Journal:Clin Cancer Res
PubMed ID:18559604
'PURPOSE: HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. We are developing molecular probes for in vivo quantitative imaging of HER2 receptors using near-infrared (NIR) optical imaging. The goal is to provide probes that will minimally interfere with the studied system, ... More
Noninvasive positron emission tomography and fluorescence imaging of CD133+ tumor stem cells.
Authors:Gaedicke S, Braun F, Prasad S, Machein M, Firat E, Hettich M, Gudihal R, Zhu X, Klingner K, Schüler J, Herold-Mende CC, Grosu AL, Behe M, Weber W, Mäcke H, Niedermann G,
Journal:
PubMed ID:24469819
'A technology that visualizes tumor stem cells with clinically relevant tracers could have a broad impact on cancer diagnosis and treatment. The AC133 epitope of CD133 currently is one of the best-characterized tumor stem cell markers for many intra- and extracranial tumor entities. Here we demonstrate the successful noninvasive detection ... More
Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.
Authors:Karlsen TV, McCormack E, Mujic M, Tenstad O, Wiig H,
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:22101523
'There is a lack of available methods to noninvasively quantify lymphatic function in small experimental animals, a necessity for studies on lymphatic system pathophysiology. We present a new method to quantify lymph flow in mice and rats, based on optically monitoring the depot clearance of near-infrared fluorescently labeled albumin and ... More
Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.
Authors:McCormack E, Mujic M, Osdal T, Bruserud Ø, Gjertsen BT,
Journal:Blood
PubMed ID:23243270
Antibodies play a fundamental role in diagnostic immunophenotyping of leukemias and in cell-targeting therapy. However, this versatility is not reflected in imaging diagnostics. In the present study, we labeled anti–human mAbs monochromatically against selected human myeloid markers expressed on acute myeloid leukemia (AML) cells, all with the same near-infrared fluorochrome. ... More
Influenza virus aerosol exposure and analytical system for ferrets.
Authors:Gustin KM, Belser JA, Wadford DA, Pearce MB, Katz JM, Tumpey TM, Maines TR,
Journal:Proc Natl Acad Sci U S A
PubMed ID:21536880
Understanding the transmission ability of newly emerging influenza viruses is central to the development of public health preparedness and prevention strategies. Animals are used to model influenza virus infection and transmission, but the routinely used intranasal inoculation of a liquid virus suspension does not reflect natural infection. We report the ... More