SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750 - FAQs

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8 product FAQs found

我该注射多少量的偶联抗体来进行肿瘤成像?

推荐的起始剂量为50 µg。您需要根据实验模型来确定最佳剂量。

我应该采用哪种规格的针头?

我们建议使用具有固定(不可移动)针头、28-32管径的结核菌素和胰岛素注射器(0.3或1.0 mL容量)。

能够注入小鼠体内的最佳和最大试剂体积是多少?

注射试剂的体积根据给药途径而异。下面是25克动物的一般指导用量:

静脉注射(IV)50–125 µL(推荐体积)- 200 µL(最大体积);腹腔注射(IP)500 µL(推荐体积)- 2 mL(最大体积);皮下注射(SC)100–250 µL(推荐体积)- 1 mL(最大体积)。

哪种类型的小鼠最适于体内成像?

由于毛皮会引起光散射,所以推荐采用无毛小鼠(如无胸腺裸鼠(nu/nu))进行体内成像。如果无法使用裸鼠,覆盖成像区域的毛发应该用剃毛机或化学脱毛剂(如Nair脱毛剂)去除。

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What size needle should I use for small animal in vivo imaging?

We recommend the use of a 28-32 gauge tuberculin or insulin syringe (0.3 or 1.0 mL volume) with a fixed (non-removable) needle.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the optimal and maximal volumes of reagent that can be injected into a mouse?

The volume of reagent that can be injected varies according to the route of administration. The following numbers are general guidelines for a 25 gram animal: Intravenous (IV)- 50-125 µl (recommended)- 200µl (maximum) ; Intraperitoneal (IP) 500µl (recommended) -2ml (maximum) ; Subcutaneous(SC) 100-250 µl (recommended)- 1ml (maximum).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of mice are the best for in vivo imaging?

Due to light scattering caused by fur, hairless mice such as athymic nude (nu/nu) mice are recommended for in vivo imaging. If this is not an option, the hair covering the area to be imaged should be removed using clippers or a chemical depilatory such as Nair depilatory.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.