INTRODUCTION: In previous studies we and others have demonstrated the usefulness of violet laser diodes (VLDs) as replacement laser sources for krypton-ion lasers on stream-in-air cytometers. Previously available VLDs had a maximum available power of less than 25 mW; this was sufficient for excitation of densely labeled cell surface antigens ... More
Novel strategy to selectively label excitatory and inhibitory neurons in the cerebral cortex of mice.
AuthorsBasu K, Gravel C, Tomioka R, Kaneko T, Tamamaki N, Sík A,
JournalJ Neurosci Methods
PubMed ID18321591
Revealing the connections of neuronal systems is critical for understanding how they function. The vast majority of neurons in all cortical areas consist of excitatory cells whose activity is controlled by inhibitory cells. Distribution and projection patterns of inhibitory and excitatory cells are key information to understand the organization of ... More
Endosomal trafficking of the ligated FcvarepsilonRI receptor.
AuthorsFattakhova GV, Masilamani M, Narayanan S, Borrego F, Gilfillan AM, Metcalfe DD, Coligan JE,
JournalMol Immunol
PubMed ID18945491
In addition to initiating signaling cascades leading to mast cell mediator release, aggregation of the high affinity IgE receptor (FcvarepsilonRI) leads to rapid internalization of the cross-linked receptor. However, little is known about the trafficking of the internalized FcvarepsilonRI. Here we demonstrate that in RBL-2H3 cells, aggregated FcvarepsilonRI appears in ... More
Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice.
AuthorsPitulescu ME, Schmidt I, Benedito R, Adams RH,
JournalNat Protoc
PubMed ID20725067
The retina is a powerful experimental system for the analysis of angiogenic blood vessel growth in the postnatal organisms. The three-dimensional architecture of the vessel network and processes as diverse as endothelial cell (EC) proliferation, sprouting, perivascular cell recruitment, vessel remodeling or maturation can be investigated at high resolution. The ... More