链霉素亲和素,Pacific Orange™ 偶联物
链霉素亲和素,Pacific Orange™ 偶联物
Invitrogen™

链霉素亲和素,Pacific Orange™ 偶联物

Pacific Orange™ 链霉素亲和素的最大吸收/发射波长分别为 ∼400/551 nm。链霉亲和素是一种非糖基化生物素结合蛋白,从细菌中分离所得,具有近中性等电点。由于经报道其非特异性结合比例比糖基化生物素结合蛋白(亲和素)更低,故广泛用于检测生物素化探针。查看所有了解更多信息
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货号数量
S323651 mg
货号 S32365
价格(CNY)
4,553.00
Each
添加至购物车
数量:
1 mg
价格(CNY)
4,553.00
Each
添加至购物车
Pacific Orange™ 链霉素亲和素的最大吸收/发射波长分别为 ∼400/551 nm。链霉亲和素是一种非糖基化生物素结合蛋白,从细菌中分离所得,具有近中性等电点。由于经报道其非特异性结合比例比糖基化生物素结合蛋白(亲和素)更低,故广泛用于检测生物素化探针。

查看所有 Alexa Fluor™ 链霉亲和素-亲和素偶联物选择指南
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
激发/发射400/551
流式细胞仪激光线路405
适用于(设备)流式细胞分析、免疫检测、组化应用、印迹分析
标签或染料Pacific Orange
产品类型链霉素亲和素偶联物(荧光)
数量1 mg
运输条件室温
偶联物Pacific Orange
形式实心
产品线Pacific Orange
Unit SizeEach
内容与储存
含 1 瓶链霉亲和素 Pacific Orange™ 偶联物。

在冰箱(-5 至 -30°C)中避光储存。

常见问题解答 (FAQ)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

引用和文献 (2)

引用和文献
Abstract
An 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood dendritic cell subsets in a single platform.
Authors:Wang JC, Kobie JJ, Zhang L, Cochran M, Mosmann TR, Ritchlin CT, Quataert SA,
Journal:J Immunol Methods
PubMed ID:19049809
Human peripheral blood dendritic cells (PBDC) are a rare population comprised of several distinctive subsets. Analysis of these cells has been hindered by their low frequency. In this study, we report a novel direct ex vivo 11-color flow cytometric assay that combines subset identification with analysis of activation status and ... More
Violet laser diodes in flow cytometry: an update.
Authors:Telford W, Kapoor V, Jackson J, Burgess W, Buller G, Hawley T, Hawley R,
Journal:Cytometry A
PubMed ID:17051581
INTRODUCTION: In previous studies we and others have demonstrated the usefulness of violet laser diodes (VLDs) as replacement laser sources for krypton-ion lasers on stream-in-air cytometers. Previously available VLDs had a maximum available power of less than 25 mW; this was sufficient for excitation of densely labeled cell surface antigens ... More