Extracellular RNA constitutes a natural procoagulant cofactor in blood coagulation.
AuthorsKannemeier C, Shibamiya A, Nakazawa F, Trusheim H, Ruppert C, Markart P, Song Y, Tzima E, Kennerknecht E, Niepmann M, von Bruehl ML, Sedding D, Massberg S, Günther A, Engelmann B, Preissner KT
JournalProc Natl Acad Sci U S A
PubMed ID17405864
'Upon vascular injury, locally controlled haemostasis prevents life-threatening blood loss and ensures wound healing. Intracellular material derived from damaged cells at these sites will become exposed to blood components and could contribute to blood coagulation and pathological thrombus formation. So far, the functional and mechanistic consequences of this concept are ... More
Cytoplasmic and intra-nuclear binding of gentamicin does not require endocytosis.
AuthorsMyrdal SE, Johnson KC, Steyger PS
JournalHear Res
PubMed ID15925201
'Understanding the cellular mechanism(s) by which the oto- and nephrotoxic aminoglycoside antibiotics penetrate cells, and the precise intracellular distribution of these molecules, will enable identification of aminoglycoside-sensitive targets, and potential uptake blockers. Clones of two kidney cell lines, OK and MDCK, were treated with the aminoglycoside gentamicin linked to the ... More
CPEB3 and CPEB4 in neurons: analysis of RNA-binding specificity and translational control of AMPA receptor GluR2 mRNA.
AuthorsHuang YS, Kan MC, Lin CL, Richter JD
JournalEMBO J
PubMed ID17024188
'CPEB is a sequence-specific RNA-binding protein that promotes polyadenylation-induced translation in oocytes and neurons. Vertebrates contain three additional genes that encode CPEB-like proteins, all of which are expressed in the brain. Here, we use SELEX, RNA structure probing, and RNA footprinting to show that CPEB and the CPEB-like proteins interact ... More
Atheroprotective communication between endothelial cells and smooth muscle cells through miRNAs.
The shear-responsive transcription factor Krüppel-like factor 2 (KLF2) is a critical regulator of endothelial gene expression patterns induced by atheroprotective flow. As microRNAs (miRNAs) post-transcriptionally control gene expression in many pathogenic and physiological processes, we investigated the regulation of miRNAs by KLF2 in endothelial cells. KLF2 binds to the promoter ... More
RNA-selective, live cell imaging probes for studying nuclear structure and function.
AuthorsLi Q, Kim Y, Namm J, Kulkarni A, Rosania GR, Ahn YH, Chang YT,
JournalChem Biol
PubMed ID16793519
The higher-order structural organization of the cell nucleus reflects the underlying genome-wide transcriptional activity and macromolecular transport processes. To study the microscopic organization of RNA distribution within the nucleus, a combinatorial library of fluorescent styryl molecules was synthesized and screened for an in vitro RNA response and live cell nuclear ... More
Genome-wide analysis demonstrates conserved localization of messenger RNAs to mitotic microtubules.
AuthorsBlower MD, Feric E, Weis K, Heald R,
JournalJ Cell Biol
PubMed ID18166649
RNA localization is of critical importance in many fundamental cell biological and developmental processes by regulating the spatial control of gene expression. To investigate how spindle-localized RNAs might influence mitosis, we comprehensively surveyed all messenger RNAs (mRNAs) that bound to microtubules during metaphase in both Xenopus laevis egg extracts and ... More
Simultaneous detection of mRNA and protein stem cell markers in live cells.
AuthorsRhee WJ, Bao G,
JournalBMC Biotechnol
PubMed ID19341452
BACKGROUND: Biological studies and medical application of stem cells often require the isolation of stem cells from a mixed cell population, including the detection of cancer stem cells in tumor tissue, and isolation of induced pluripotent stem cells after eliciting the expression of specific genes in adult cells. Here we ... More
Sequential treatment of drug-resistant tumors with targeted minicells containing siRNA or a cytotoxic drug.
The dose-limiting toxicity of chemotherapeutics, heterogeneity and drug resistance of cancer cells, and difficulties of targeted delivery to tumors all pose daunting challenges to effective cancer therapy. We report that small interfering RNA (siRNA) duplexes readily penetrate intact bacterially derived minicells previously shown to cause tumor stabilization and regression when ... More
Lipophilic dye staining of Cryptococcus neoformans extracellular vesicles and capsule.
AuthorsNicola AM, Frases S, Casadevall A,
JournalEukaryot Cell
PubMed ID19465562
Cryptococcus neoformans is an encapsulated yeast that causes systemic mycosis in immunosuppressed individuals. Recent studies have determined that this fungus produces vesicles that are released to the extracellular environment both in vivo and in vitro. These vesicles contain assorted cargo that includes several molecules associated with virulence and implicated in ... More
A role for huntington disease protein in dendritic RNA granules.
AuthorsSavas JN, Ma B, Deinhardt K, Culver BP, Restituito S, Wu L, Belasco JG, Chao MV, Tanese N,
JournalJ Biol Chem
PubMed ID20185826
Regulated transport and local translation of mRNA in neurons are critical for modulating synaptic strength, maintaining proper neural circuitry, and establishing long term memory. Neuronal RNA granules are ribonucleoprotein particles that serve to transport mRNA along microtubules and control local protein synthesis in response to synaptic activity. Studies suggest that ... More
Exploiting nuclear duality of ciliates to analyse topological requirements for DNA replication and transcription.
AuthorsPostberg J, Alexandrova O, Cremer T, Lipps HJ
JournalJ Cell Sci
PubMed ID16129882
Spatial and temporal replication patterns are used to describe higher-order chromatin organisation from nuclei of early metazoan to mammalian cells. Here we demonstrate evolutionary conserved similarities and differences in replication patterns of micronuclei and macronuclei in the spirotrichous ciliate Stylonychia lemnae. Since this organism possesses two kinds of morphologically and ... More
RNA Binding Antagonizes Neurotoxic Phase Transitions of TDP-43.
'TDP-43 proteinopathy is a pathological hallmark of amyotrophic lateral sclerosis and frontotemporal dementia where cytoplasmic TDP-43 inclusions are observed within degenerating regions of patient postmortem tissue. The mechanism by which TDP-43 aggregates has remained elusive due to technological limitations, which prevent the analysis of specific TDP-43 interactions in live cells. ... More
T-cell trans-synaptic vesicles are distinct and carry greater effector content than constitutive extracellular vesicles.
Authors
JournalNat Commun
PubMed ID35710644
An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.
AuthorsClancy JW, Zhang Y, Sheehan C, D'Souza-Schorey C
JournalNat Cell Biol
PubMed ID31235936
'Tumour-derived microvesicles (TMVs) comprise a class of extracellular vesicles released from tumour cells that are now understood to facilitate communication between the tumour and the surrounding microenvironment. Despite their significance, the regulatory mechanisms governing the trafficking of bioactive cargos to TMVs at the cell surface remain poorly defined. Here we ... More
Carbon dot-protoporphyrin IX conjugates for improved drug delivery and bioimaging.
AuthorsAguilar Cosme JR, Bryant HE, Claeyssens F
JournalPLoS One
PubMed ID31344086
'Photodynamic therapy (PDT) uses photosensitisers such as protoporphyrin IX (PpIX) to target tumours via the release of toxic singlet oxygen when irradiated. The effectivity of the treatment is limited by the innate properties of the photosensitizers; they typically exhibit inefficient accumulation in target tissue and high dark toxicity. Carbon dots ... More
Neutrophil extracellular trap-associated RNA and LL37 enable self-amplifying inflammation in psoriasis.
'Psoriasis is an inflammatory skin disease with strong neutrophil (PMN) infiltration and high levels of the antimicrobial peptide, LL37. LL37 in complex with DNA and RNA is thought to initiate disease exacerbation via plasmacytoid dendritic cells. However, the source of nucleic acids supposed to start this initial inflammatory event remains ... More
SUPT4H1 Depletion Leads to a Global Reduction in RNA.
AuthorsNaguib A, Sandmann T, Yi F, Watts RJ, Lewcock JW, Dowdle WE
JournalCell Rep
PubMed ID30605685
'SUPT4H1 is a transcription elongation factor that makes up part of the RNA polymerase II complex. Recent studies propose a selective role for SUPT4H1 in the transcription of repeat-containing DNA, the translated products of which contribute to neurodegenerative disorders such as C9orf72-amyotrophic lateral sclerosis. To investigate the potential of SUPT4H1 ... More
NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility.
Authors
JournalNat Commun
PubMed ID35697676
MeCP2 Levels Regulate the 3D Structure of Heterochromatic Foci in Mouse Neurons.
Authors
JournalJ Neurosci
PubMed ID33046553
FMRP-PKA Activity Negative Feedback Regulates RNA Binding-Dependent Fibrillation in Brain Learning and Memory Circuitry.
Authors
JournalCell Rep
PubMed ID33053340
Extremely Low Forces Induce Extreme Axon Growth.
Authors
JournalJ Neurosci
PubMed ID32444384
Evidence for autophagy-dependent pathways of rRNA turnover in Arabidopsis.
Authors
JournalAutophagy
PubMed ID26735434
Dynamic assembly of the mRNA m6A methyltransferase complex is regulated by METTL3 phase separation.
Authors
JournalPLoS Biol
PubMed ID35143475
Early nucleolar disorganization in Dictyostelium cell death.
Authors
JournalCell Death Dis
PubMed ID28055008
Exchange of cellular components between platelets and tumor cells: impact on tumor cells behavior.
Authors
JournalTheranostics
PubMed ID35265204
Cell entry of a host-targeting protein of oomycetes requires gp96.
Authors
JournalNat Commun
PubMed ID29904064
High Levels of miR-483-3p Are Present in Serum Exosomes Upon Infection of Mice With Highly Pathogenic Avian Influenza Virus.
AuthorsMaemura T, Fukuyama S, Kawaoka Y
JournalFront Microbiol
PubMed ID32117163
Exosomes, the extracellular vesicles that contain functional proteins and RNAs, regulate cell-cell communication. Recently, our group reported that levels of various microRNAs (miRNAs) in bronchoalveolar lavage fluid exosomes were highly increased in influenza virus-infected mice and that one of those miRNAs, miR-483-3p, was involved in the potentiation of the innate ... More
Loss of p53 drives neuron reprogramming in head and neck cancer.
AuthorsAmit M, Takahashi H, Dragomir MP, Lindemann A, Gleber-Netto FO, Pickering CR, Anfossi S, Osman AA, Cai Y, Wang R, Knutsen E, Shimizu M, Ivan C, Rao X, Wang J, Silverman DA, Tam S, Zhao M, Caulin C, Zinger A, Tasciotti E, Dougherty PM, El-Naggar A, Calin GA, Myers JN
JournalNature
PubMed ID32051587
The solid tumour microenvironment includes nerve fibres that arise from the peripheral nervous system
Modulation of RNA Condensation by the DEAD-Box Protein eIF4A.
AuthorsTauber D, Tauber G, Khong A, Van Treeck B, Pelletier J, Parker R
JournalCell
PubMed ID31928844
Stress granules are condensates of non-translating mRNAs and proteins involved in the stress response and neurodegenerative diseases. Stress granules form in part through intermolecular RNA-RNA interactions, and to better understand how RNA-based condensation occurs, we demonstrate that RNA is effectively recruited to the surfaces of RNA or RNP condensates in vitro. We ... More