Protein kinase C-beta II Is an apoptotic lamin kinase in polyomavirus-transformed, etoposide-treated pyF111 rat fibroblasts.
AuthorsChiarini A, Whitfield JF, Armato U, Dal Pra I,
JournalJ Biol Chem
PubMed ID11901153
'The role of protein kinase C-beta(II) (PKC-beta(II)) in etoposide (VP-16)-induced apoptosis was studied using polyomavirus-transformed pyF111 rat fibroblasts in which PKC-beta(II) specific activity in the nuclear membrane (NM) doubled and the enzyme was cleaved into catalytic fragments. No PKC-beta(II) complexes with lamin B1 and/or active caspases were immunoprecipitable from the ... More
Osmosensitive taurine transporter expression and activity in human corneal epithelial cells.
AuthorsShioda R, Reinach PS, Hisatsune T, Miyamoto Y,
JournalInvest Ophthalmol Vis Sci
PubMed ID12202510
'To characterize in SV40-immortalized human corneal epithelial cells (tHCEC) osmosensitive taurine transporter gene and protein expression as well as its functional activity. To evaluate whether medium supplementation with taurine improves cell viability during a hypertonic challenge. tHCEC were preincubated for up to 48 hours in hypertonic DMEM medium (i.e., up ... More
Survival of chondrocytes in rabbit septal cartilage after electromechanical reshaping.
AuthorsProtsenko DE, Ho K, Wong BJ,
JournalAnn Biomed Eng
PubMed ID20842431
'Electromechanical reshaping (EMR) has been recently described as an alternative method for reshaping facial cartilage without the need for incisions or sutures. This study focuses on determining the short- and long-term viability of chondrocytes following EMR in cartilage grafts maintained in tissue culture. Flat rabbit nasal septal cartilage specimens were ... More
Damage to developing mouse skeletal muscle myotubes in culture: protective effect of heat shock proteins.
AuthorsMaglara AA, Vasilaki A, Jackson MJ, McArdle A,
JournalJ Physiol
PubMed ID12598587
'Damage to skeletal muscle occurs following excessive exercise, upon reperfusion following ischaemia and in disease states, such as muscular dystrophy. Key mechanisms by which damage is thought to occur include a loss of intracellular calcium homeostasis, loss of energy supply to the cell, increased activity of oxidising free radical-mediated reactions ... More
Graphene microsheets enter cells through spontaneous membrane penetration at edge asperities and corner sites.
AuthorsLi Y, Yuan H, von dem Bussche A, Creighton M, Hurt RH, Kane AB, Gao H,
Journal
PubMed ID23840061
'Understanding and controlling the interaction of graphene-based materials with cell membranes is key to the development of graphene-enabled biomedical technologies and to the management of graphene health and safety issues. Very little is known about the fundamental behavior of cell membranes exposed to ultrathin 2D synthetic materials. Here we investigate ... More
Engineering an in situ crosslinkable hydrogel for enhanced remyelination.
AuthorsLi X, Liu X, Cui L, Brunson C, Zhao W, Bhat NR, Zhang N, Wen X,
JournalFASEB J
PubMed ID23239823
'Remyelination has to occur to fully regenerate injured spinal cords or brain tissues. A growing body of evidence has suggested that exogenous cell transplantation is one promising strategy to promote remyelination. However, direct injection of neural stem cells or oligodendrocyte progenitor cells (OPCs) to the lesion site may not be ... More
Targeted deletion of Puma attenuates cardiomyocyte death and improves cardiac function during ischemia-reperfusion.
AuthorsToth A, Jeffers JR, Nickson P, Min JY, Morgan JP, Zambetti GP, Erhardt P,
JournalAm J Physiol Heart Circ Physiol
PubMed ID16399862
The p53-upregulated modulator of apoptosis (Puma), a BH3-only member of the Bcl-2 protein family, is required for p53-dependent and -independent forms of apoptosis and has been implicated in the pathomechanism of several diseases, including cancer, acquired immunodeficiency syndrome, and ischemic brain disease. The role of Puma in cardiomyocyte death, however, ... More
Tyrosine phosphorylation regulates the proteolytic activation of protein kinase Cdelta in dopaminergic neuronal cells.
AuthorsKaul S, Anantharam V, Yang Y, Choi CJ, Kanthasamy A, Kanthasamy AG,
JournalJ Biol Chem
PubMed ID15961393
Oxidative stress is a key apoptotic stimulus in neuronal cell death and has been implicated in the pathogenesis of many neurodegenerative disorders, including Parkinson disease (PD). Recently, we demonstrated that protein kinase C-delta (PKCdelta) is an oxidative stress-sensitive kinase that can be activated by caspase-3-dependent proteolytic cleavage to induce apoptotic ... More
Hepatocyte growth factor activator inhibitor-2 (HAI-2) is a favorable prognosis marker and inhibits cell growth through the apoptotic pathway in cervical cancer.
AuthorsNakamura K, Abarzua F, Hongo A, Kodama J, Nasu Y, Kumon H, Hiramatsu Y,
JournalAnn Oncol
PubMed ID18689863
In light of the poor prognosis for cervical cancer, research continues into the development of innovative and efficacious treatment modalities for this disease. We investigated the role of hepatocyte growth factor activator inhibitor-2 (HAI-2) and evaluated its clinical importance in cervical cancer. HAI-2 expression was examined in cervical cancer specimens ... More
Cytosolic recognition of flagellin by mouse macrophages restricts Legionella pneumophila infection.
AuthorsMolofsky AB, Byrne BG, Whitfield NN, Madigan CA, Fuse ET, Tateda K, Swanson MS,
JournalJ Exp Med
PubMed ID16606669
To restrict infection by Legionella pneumophila, mouse macrophages require Naip5, a member of the nucleotide-binding oligomerization domain leucine-rich repeat family of pattern recognition receptors, which detect cytoplasmic microbial products. We report that mouse macrophages restricted L. pneumophila replication and initiated a proinflammatory program of cell death when flagellin contaminated their ... More
Trehalose protects corneal epithelial cells from death by drying.
AuthorsMatsuo T,
JournalBr J Ophthalmol
PubMed ID11316726
This study was designed to examine whether trehalose could protect corneal epithelial cells in culture from death by desiccation in order to test trehalose as a potential new eye drop for dry eye syndrome. Human corneal epithelial cells in near confluent culture in wells of a 96 well multidish were ... More