SYTO™ RNASelect™ Red, 1 mL - FAQs

How can I prepare my exosome sample (isolated using the Total Exosome Isolation reagent) for analysis by mass spectroscopy?

The Total Exosome Isolation reagent allows recovery of the entire exosome population from the sample, in contrast to ultracentrifugation protocols, which recover significantly less material. Thus, the pellet is larger. The purity of exosomes is very similar for cell media samples (reagent vs ultra); for more challenging samples such as serum (which are more viscous and have much higher protein content, etc.) the reagent recovers exosomes at a lower purity (few microvesicles and large protein complexes co-precipitate) compared to ultracentrifugation. Having said this, you can obtain enough material for all standard types of downstream analysis, such as qRT-PCR or Western blot with either method.